Investigation of the retinal biocompatibility of acid violet for chromovitrectomy

Investigation of the retinal biocompatibility of acid violet for chromovitrectomy

Autor Cardoso, Emmerson Badaro Autor UNIFESP Google Scholar
Moraes-Filho, Milton Autor UNIFESP Google Scholar
Rodrigues, Eduardo B. Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Penha, Fernando M. Autor UNIFESP Google Scholar
Novais, Eduardo Amorim Autor UNIFESP Google Scholar
Souza-Lima, Rodrigo A. Autor UNIFESP Google Scholar
Meyer, Carsten H. Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Bonn
Resumo The primary objective was to investigate the retinal biocompatibility of acid violet (AV) as a vital dye for chromovitrectomy. the secondary objective was to evaluate the capacity of AV to stain the anterior capsule of the lens.An amount of 0.05 ml of 0.25 g/l and 0.5 g/l AV was injected intravitreally in the OD, while balanced salt solution (BSS) was applied in the OS for control. Clinical examination and histology with light microscopy (LM) were performed after 7 days. Retinal cell layers were evaluated for morphologic alterations and number of cells. the electroretinographic (ERG) changes were assessed at baseline and 7 days. in another part of the study, 0.1 ml of 0.25 g/l AV was injected into the anterior chamber of ex-vivo porcine eyes, and its capacity to stain the anterior capsule was determined. Cadaveric eyes were used to test the capacity of AV to stain the internal limitant membrane (ILM) during vitrectomy.The gross histopathologic appearance of the retina, choroids, sclera, and optic nerve was within normal limits, without any signs of severe retinal necrosis or cystic degeneration. AV caused no substantial retinal alterations in retinal layers by LM at either the lower or higher dose when compared with the control eye. the injection of AV did not induce considerable ERG alterations. the violet dye stained the anterior capsule after anterior chamber injection and the ILM, allowing a safer capsulorrhexis and vitrectomy.Acid violet may be safe for the retina at concentrations of 0.25 and 0.50 g/l after intravitreous injection, and may be used as a vital dye for staining the anterior capsule and the ILM.
Palavra-chave Chromovitrectomy
Acid violet
Vitreoretinal dyes
Retinal toxicity
Electroretinogram
Intraocular toxicity
Idioma Inglês
Financiador German Academic Exchange Service (DAAD)
Pan-American Association of Ophthalmology-Pan-American Ophthalmological Foundation (PAAO/PAOF)
Vision Institute - UNIFESP
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Data de publicação 2013-04-01
Publicado em Graefes Archive for Clinical and Experimental Ophthalmology. New York: Springer, v. 251, n. 4, p. 1115-1121, 2013.
ISSN 0721-832X (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 1115-1121
Fonte http://dx.doi.org/10.1007/s00417-013-2258-y
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000316759000010
Endereço permanente http://repositorio.unifesp.br/handle/11600/36144

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