Identification of human plasma proteins associated with the cell wall of the pathogenic fungus Paracoccidioides brasiliensis

Identification of human plasma proteins associated with the cell wall of the pathogenic fungus Paracoccidioides brasiliensis

Autor Longo, Larissa V. G. Autor UNIFESP Google Scholar
Nakayasu, Ernesto S. Google Scholar
Matsuo, Alisson L. Autor UNIFESP Google Scholar
Silva, Roberta Peres da Autor UNIFESP Google Scholar
Sobreira, Tiago J. P. Google Scholar
Vallejo, Milene C. Autor UNIFESP Google Scholar
Ganiko, Luciane Google Scholar
Almeida, Igor C. Google Scholar
Puccia, Rosana Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Texas El Paso
Natl Ctr Res Energy & Mat
Resumo Paracoccidioides brasiliensis and Paracoccidioides lutzii are thermodimorphic species that cause paracoccidioidomycosis. the cell wall is the outermost fungal organelle to form an interface with the host. A number of host effector compounds, including immunologically active molecules, circulate in the plasma. in the present work, we extracted cell-wall-associated proteins from the yeast pathogenic phase of P.brasiliensis, isolate Pb3, grown in the presence of human plasma and analyzed bound plasma proteins by liquid chromatographytandem mass spectrometry. Transport, complement activation/regulation, and coagulation pathway were the most abundant functional groups identified. Proteins related to iron/copper acquisition, immunoglobulins, and protease inhibitors were also detected. Several human plasma proteins described here have not been previously reported as interacting with fungal components, specifically, clusterin, hemopexin, transthyretin, ceruloplasmin, alpha-1-antitrypsin, apolipoprotein A-I, and apolipoprotein B-100. Additionally, we observed increased phagocytosis by J774.16 macrophages of Pb3 grown in plasma, suggesting that plasma proteins interacting with P.brasiliensis cell wall might be interfering in the fungal relationship with the host.
Palavra-chave Paracoccidioides brasiliensis
cell wall
human plasma proteins
Idioma Inglês
Data de publicação 2013-04-01
Publicado em Fems Microbiology Letters. Hoboken: Wiley-Blackwell, v. 341, n. 2, p. 87-95, 2013.
ISSN 0378-1097 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 87-95
Fonte http://dx.doi.org/10.1111/1574-6968.12097
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000316911800003
Endereço permanente http://repositorio.unifesp.br/handle/11600/36115

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