Antibodies to intimin and Escherichia coli-secreted proteins EspA and EspB in sera of Brazilian children with hemolytic uremic syndrome and healthy controls

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dc.contributor.author Guirro, Mirian [UNIFESP]
dc.contributor.author Souza, Renato Lopes de [UNIFESP]
dc.contributor.author Piazza, Roxane M. F.
dc.contributor.author Guth, Beatriz E. C. [UNIFESP]
dc.date.accessioned 2016-01-24T14:31:25Z
dc.date.available 2016-01-24T14:31:25Z
dc.date.issued 2013-03-15
dc.identifier http://dx.doi.org/10.1016/j.vetimm.2012.09.016
dc.identifier.citation Veterinary Immunology and Immunopathology. Amsterdam: Elsevier B.V., v. 152, n. 1-2, p. 121-125, 2013.
dc.identifier.issn 0165-2427
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36089
dc.description.abstract Enterohemorrhagic Escherichia coli (EHEC) strains can cause hemolytic uremic syndrome (HUS), a leading cause of childhood renal failure. the adhesin intimin and the secreted proteins A (EspA) and B (EspB) contribute to the occurrence of EHEC attaching and effacing lesions. in this study, immunoblot assays were performed to determine immunoglobulin G (IgG) antibodies reactive with these proteins in sera from 13 children diagnosed with HUS and in sera from 54 healthy Brazilian children. in general, high frequencies of serum IgG antibodies reactive with EspA, EspB and the conserved region of intimin were observed in both HUS patients and controls with no statistically significant differences. However, a marked difference in immune response to these proteins was observed in HUS patients compared to controls in infants less than two years of age. in addition, IgG against the variable region of intimin was more frequently detected in HUS patients than in children with no signs of infection (p < 0.05) regardless of age, suggesting that the detection of antibodies directed to the variable region of intimin gamma can be useful in serodiagnostic tests of EHEC-infected patients. the immune response against intimin and structural proteins encoded by the locus of enterocyte effacement pathogenicity island in patients with HUS has previously not been described in Brazil. the results presented here may contribute to the development of diagnostic tools and complement information concerning EHEC epidemiology in our setting. (C) 2012 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 121-125
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Veterinary Immunology and Immunopathology
dc.rights Acesso restrito
dc.subject Antibodies en
dc.subject Hemolytic uremic syndrome en
dc.subject Children en
dc.subject Intimin en
dc.subject EspA en
dc.subject EspB en
dc.title Antibodies to intimin and Escherichia coli-secreted proteins EspA and EspB in sera of Brazilian children with hemolytic uremic syndrome and healthy controls en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Inst Butantan
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Pediat, Pediat Intens Care Unit, São Paulo, Brazil
dc.description.affiliation Inst Butantan, Bacteriol Lab, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Pediat, Pediat Intens Care Unit, São Paulo, Brazil
dc.description.sponsorshipID CNPq: 142458/2007-4
dc.identifier.doi 10.1016/j.vetimm.2012.09.016
dc.description.source Web of Science
dc.identifier.wos WOS:000316535000017



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