Evaluation of 16 SNPs allele-specific to quantify post hSCT chimerism by SYBR green-based qRT-PCR

Evaluation of 16 SNPs allele-specific to quantify post hSCT chimerism by SYBR green-based qRT-PCR

Author Cardoso Almeida, Carlos Arthur Autor UNIFESP Google Scholar
Dreyfuss, Juliana Luporini Autor UNIFESP Google Scholar
Azevedo-Shimmoto, Marily Maria Autor UNIFESP Google Scholar
Figueiredo, Maria Stela Autor UNIFESP Google Scholar
Rodrigues de Oliveira, Jose Salvador Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Alagoas UFAL
Santa Marcelina Hosp
Abstract The importance of monitoring post haematopoietic stem cell transplantation (hSCT) chimerism has been defined in numerous publications. Single-nucleotide polymorphisms (SNPs) are molecular markers that vary significantly among different populations. Allied to a very sensible technique, SNP assays seem to be very sensitive (0.001%) when post hSCT chimerism is measured. However, well known SNP frequencies are limited to certain populations, mainly in countries where there is a high level of diversity in its population, therefore restricting their use worldwide. Amplification by SYBR green based quantitative real time PCR of eight pairs of allele-specific SNPs (MLH-1, PECAM-1, ICAM-1, SUR-1, HA-1, rs715405, rs713503, rs2296600) was conducted in 88 patient/donor pairs, who underwent allogeneic myeloablative or non-myeloablative hSCT. One informative allele was detected in at least 42% (n=37) of the samples; 20% (n=18) had at least two informative alleles; 10% (n=9) had at least three informative alleles; 9% (n=8) had more than three informative alleles and 18% (n=16) showed no informative allele at all. Overall, the frequency of informative alleles for these SNPs in the Brazilian population was very low. Consequently, the amount of information attained reached 9% of those expected, being able to discriminate only eight pairs of donor/recipient samples with more than three informative alleles, making them useless for the quantification of chimerism in our routine.
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-03-01
Published in Journal of Clinical Pathology. London: Bmj Publishing Group, v. 66, n. 3, p. 238-242, 2013.
ISSN 0021-9746 (Sherpa/Romeo, impact factor)
Publisher Bmj Publishing Group
Extent 238-242
Origin http://dx.doi.org/10.1136/jclinpath-2012-201224
Access rights Closed access
Type Article
Web of Science ID WOS:000315292200012
URI http://repositorio.unifesp.br/handle/11600/36065

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