Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats

Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats

Author Fernandes, Kelly Rossetti Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Rodrigues, Natalia Camargo Autor UNIFESP Google Scholar
Tim, Carla Autor UNIFESP Google Scholar
Santos, Anderson Amaro Autor UNIFESP Google Scholar
Parizotto, Nivaldo Antonio Autor UNIFESP Google Scholar
Araujo, Heloisa Selistre de Autor UNIFESP Google Scholar
Driusso, Patricia Autor UNIFESP Google Scholar
Renno, Ana Claudia Muniz Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We evaluate the effects of low-level laser therapy (LLLT) on the histological modifications and temporal osteogenic genes expression during the initial phase of bone healing in a model of bone defect in rats. Sixty-four Wistar rats were divided into control and treated groups. Noncritical size bone defects were surgically created at the upper third of the tibia. Laser irradiation (Ga-Al-As laser 830 nm, 30 mW, 0.028 cm(2), 1.071 W/cm(2), 1 min and 34 s, 2.8 Joules, 100 J/cm(2)) was performed for 1, 2, 3, and 5 sessions. Histopathology revealed that treated animals presented higher inflammatory cells recruitment, especially 12 and 36 h postsurgery. Also, a better tissue organization at the site of the injury, with the presence of granulation tissue and new bone formation was observed on days three and five postsurgery in the treated animals. the quantitative real time polymerase chain reaction showed that LLLT produced a significantly increase in mRNA expression of Runx-2, 12 h and three days post-surgery, a significant upregulation of alkaline phosphatase mRNA expression after 36 h and three days post-surgery and a significant increase of osteocalcin mRNA expression after three and five days. We concluded that LLLT modulated the inflammatory process and accelerated bone repair, and this advanced repair pattern in the laser-treated groups may be related to the higher mRNA expression of genes presented by these animals. (C) 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Keywords Rats
Bone regeneration
Low-level laser therapy
Genetic analysis
Language English
Date 2013-03-01
Published in Journal of Biomedical Optics. Bellingham: Spie-soc Photo-optical Instrumentation Engineers, v. 18, n. 3, 6 p., 2013.
ISSN 1083-3668 (Sherpa/Romeo, impact factor)
Publisher Spie-soc Photo-optical Instrumentation Engineers
Extent 6
Origin https://doi.org/10.1117/1.JBO.18.3.038002
Access rights Closed access
Type Article
Web of Science ID WOS:000322336700041
URI https://repositorio.unifesp.br/handle/11600/36063

Show full item record


File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)




My Account