Characterization of Novel O-Glycans Isolated from Tear and Saliva of Ocular Rosacea Patients

Characterization of Novel O-Glycans Isolated from Tear and Saliva of Ocular Rosacea Patients

Author Ozcan, Sureyya Google Scholar
An, Hyun Joo Google Scholar
Vieira, Ana C. Autor UNIFESP Google Scholar
Park, Gun Wook Google Scholar
Kim, Jae Han Google Scholar
Mannis, Mark J. Google Scholar
Lebrilla, Carlito B. Google Scholar
Institution Univ Calif Davis
Chungnam Natl Univ
Univ Calif
Universidade Federal de São Paulo (UNIFESP)
Abstract O-Glycans in saliva and tear isolated from patients suffering from ocular rosacea, a form of inflammatory ocular surface disease, were profiled, and their structures were elucidated using high resolution mass spectrometry. We have previously shown that certain structures, particularly sulfated oligosaccharides, increased in the tear and saliva of rosacea patients in this study, the structures of these glycans were elucidated using primarily tandem mass spectrometry. There were important similarities in the glycan profiles of tears and saliva with the majority of the structures in common. the structures of the most abundant species common to both tear and saliva, which were also the most abundant species in both, were elucidated. for sulfated species, the positions of the sulfate groups were localized. the majority of the structures were new, with the sulfated glycans comprising mucin core 1- and core 2-type structures. As both saliva and tear are rich in mucins, it is suggested that the O-glycans are mainly components of mucins. the study further illustrates the strong correspondence between the glycans in the tear and saliva of ocular rosacea patients.
Keywords O-glycan
saliva
tear
mass spectrometry
structural characterization
sulfated O-glycan
sialylated O-glycan
Language English
Sponsor National Institutes of Health
Converging Research Center Program through the Ministry of Education, Science and Technology
Grant number National Institutes of Health: RO1GM049077
Converging Research Center Program through the Ministry of Education, Science and Technology: 2011K000968
Date 2013-03-01
Published in Journal of Proteome Research. Washington: Amer Chemical Soc, v. 12, n. 3, p. 1090-1100, 2013.
ISSN 1535-3893 (Sherpa/Romeo, impact factor)
Publisher Amer Chemical Soc
Extent 1090-1100
Origin http://dx.doi.org/10.1021/pr3008013
Access rights Closed access
Type Article
Web of Science ID WOS:000315708000003
URI http://repositorio.unifesp.br/handle/11600/36043

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