An evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films

An evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films

Author Antonioli, Eliane Google Scholar
Lobo, Anderson O. Google Scholar
Ferretti, Mario Autor UNIFESP Google Scholar
Cohen, Moises Autor UNIFESP Google Scholar
Marciano, Fernanda R. Google Scholar
Corat, Evaldo J. Google Scholar
Trava-Airoldi, Vladimir J. Google Scholar
Institution Hosp Israelita Albert Einstein
Univ Vale Paraiba
Universidade Federal de São Paulo (UNIFESP)
Inst Nacl Pesquisas Espaciais
Abstract Cartilage serves as a low-friction and wear-resistant articulating surface in diarthrodial joints and is also important during early stages of bone remodeling. Recently, regenerative cartilage research has focused on combinations of cells paired with scaffolds. Superhydrophilic vertically aligned carbon nanotubes (VACNTs) are of particular interest in regenerative medicine. the aim of this study is to evaluate cell expansion of human articular chondrocytes on superhydrophilic VACNTs, as well as their morphology and gene expression. VACNT films were produced using a microwave plasma chamber on Ti substrates and submitted to an O-2 plasma treatment to make them superhydrophilic. Human chondrocytes were cultivated on superhydrophilic VACNTs up to five days. Quantitative RT-PCR was performed to measure type I and type II Collagen, Sox9, and Aggrecan mRNA expression levels. the morphology was analyzed by scanning electron microscopy (SEM) and confocal microscopy. SEM images demonstrated that superhydrophilic VACNTs permit cell growth and adhesion of human chondrocytes. the chondrocytes had an elongated morphology with some prolongations. Chondrocytes cultivated on superhydrophilic VACNTs maintain the level expression of Aggrecan, Sox9, and Collagen II determined by gPCR. This study was the first to indicate that superhydrophilic VACNTs may be used as an efficient scaffold for cartilage or bone repair. (C) 2012 Elsevier B.V. All rights reserved.
Keywords Multiwalled carbon nanotubes
Vertically aligned
Endochondral ossification
Gene expression
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: Proc: 2011/17877-7
FAPESP: 2011/20345-7
Date 2013-03-01
Published in Materials Science & Engineering C-materials for Biological Applications. Amsterdam: Elsevier B.V., v. 33, n. 2, p. 641-647, 2013.
ISSN 0928-4931 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 641-647
Access rights Closed access
Type Article
Web of Science ID WOS:000315761700011

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