Effect of transgastric peritoneal access on peritoneal innate cellular immunity: experimental study in swine

Effect of transgastric peritoneal access on peritoneal innate cellular immunity: experimental study in swine

Author Rodrigues, Rodrigo Autor UNIFESP Google Scholar
Rezende, Marcelo Autor UNIFESP Google Scholar
Gomes, Gustavo Autor UNIFESP Google Scholar
Souza, Fernando Google Scholar
Blagitz, Maiara Google Scholar
Della Libera, Alice Google Scholar
Taha, Murched Autor UNIFESP Google Scholar
Ferrari, Angelo Paulo Autor UNIFESP Google Scholar
Della Libera, Ermelindo Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fleury Med & Saude
Hosp Israelita Albert Einstein
Abstract One of the main concerns of natural orifice surgery is the local and systemic impact on physiology. Few studies have compared natural orifice transluminal endoscopic surgery (NOTES) with other surgical modalities. Most studies are based on systemic variables such as postoperative serum cytokines, with conflicting results. Surgical trauma induces an early inflammatory response, release of cytokines, and local leukocyte activation and oxidative burst. Major surgical trauma is related to impairment of phagocytic function and an increase in production of active oxygen species by phagocytes. the aim of this study was to evaluate the impact of transgastric peritoneoscopy on peritoneal innate immune response compared with laparoscopy and laparotomy in swine.Thirty-four male Sus scrofa domesticus swine were assigned to four groups: transgastric peritoneoscopy (13), laparoscopy (7), laparotomy (7), and sham procedure (7). Twenty-four hours after the procedure, peritoneal fluid cells were harvested by peritoneal washing after necropsy. Flow cytometry analysis of labeled S. aureus and E. coli phagocytosis by peritoneal neutrophils and macrophages was blindly performed. Oxidative burst activity measured by H2O2 production under different challenges was also evaluated.Total operative time varied between all groups. the transgastric, laparoscopy, and laparotomy groups required 56, 17.2, and 40.3 min of mean operative time, respectively (p < 0.05). Even though the mean percentage and intensity of phagocytosis by peritoneal phagocytes were higher in the sham, transgastric, and laparoscopy groups, there was no significant difference between these groups and laparotomy. Macrophage production of H2O2 has been shown to be similar among the transgastric, laparoscopy, and sham groups, and smaller than that in laparotomy (p < 0.05), either under basal conditions, while performing E. coli phagocytosis, or challenged by the presence of E. coli membrane lipopolysaccharide.Under the conditions of this study, transgastric peritoneoscopy has been shown to have minimal impact on peritoneal innate immune response.
Keywords Natural orifice endoscopic surgery
Laparoscopy
Immunity
Innate
Flow cytometry
Free radicals
Peritoneum
Language English
Date 2013-03-01
Published in Surgical Endoscopy and Other Interventional Techniques. New York: Springer, v. 27, n. 3, p. 964-970, 2013.
ISSN 0930-2794 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 964-970
Origin http://dx.doi.org/10.1007/s00464-012-2541-8
Access rights Closed access
Type Article
Web of Science ID WOS:000315145500036
URI http://repositorio.unifesp.br/handle/11600/36023

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