RHD gene polymorphisms in alloimmunized RhD-negative individuals with high rate of racial admixture

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dc.contributor.author Martin, Fabio O. [UNIFESP]
dc.contributor.author Menezes, Sidneia S. de
dc.contributor.author Chiba, Akemi K. [UNIFESP]
dc.contributor.author Langhi, Dante M.
dc.contributor.author Nardozza, Luciano M. M. [UNIFESP]
dc.contributor.author Chiattone, Carlos S.
dc.contributor.author Bordin, Jose O. [UNIFESP]
dc.date.accessioned 2016-01-24T14:31:14Z
dc.date.available 2016-01-24T14:31:14Z
dc.date.issued 2013-02-01
dc.identifier http://dx.doi.org/10.1016/j.transci.2012.09.004
dc.identifier.citation Transfusion and Apheresis Science. Oxford: Pergamon-Elsevier B.V., v. 48, n. 1, p. 113-116, 2013.
dc.identifier.issn 1473-0502
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35943
dc.description.abstract Background: the D-negative phenotype is the result of the total RHD gene deletion in almost all Caucasians, but it accounts for only about 20% in Africans and 70% in Asians. in Africans the RHD Psi that is one of the most important causes of the D-negative phenotype. We investigated the RHD polymorphisms in D-negative phenotype mixed Brazilians who have anti-D alloantibody.Study design and methods: Blood samples from 130 individuals previously typed as D-negative were phenotyped again using: (a) two tube reagents (Anti-D blend reagent, Cellular line TH-28, MS-26; and Anti-D polyclonal); (b) one gel test ID-Card for Rh subgroups including C-w and K antigen; and (c) ABO/Rh (Anti-D blend reagent, Cellular line 175-2, LDM3). the method used for RHD screening detected the presence of RHD exon 10 and intron 4. Sequence analysis was performed on PCR products amplified from genomic DNA for all 10 exons RHD gene.Results: We found that 1181130 (90.8%) of D-negative tested individuals had total RHD gene deletion, while 12/130 (9.2%) showed RHD gene polymorphisms. the RHD Psi was found in 10 (7.7%) individuals, one sample (0.77%) hybrid RHD-CE-D-s /RHD Psi, and another (0.77%) weak D type 4.2.Conclusions: the results showed that the RHD gene was present in 9.2% of racially mixed Brazilians who produced usually clinically significant anti-D alloantibodies. Therefore, the data showed that careful attention is necessary for clinicians in applying RhD genotyping to transfusion medicine in populations with high rate of racial admixture. (C) 2012 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 113-116
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Transfusion and Apheresis Science
dc.rights Acesso restrito
dc.subject RHD gene en
dc.subject Polymorphisms en
dc.subject Anti-D alloantibody en
dc.title RHD gene polymorphisms in alloimmunized RhD-negative individuals with high rate of racial admixture en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Fac Ciencias Med Santa Casa São Paulo
dc.description.affiliation Universidade Federal de São Paulo, Disciplina Hematol & Hemoterapia, São Paulo, Brazil
dc.description.affiliation Fac Ciencias Med Santa Casa São Paulo, Disciplina Hematol & Hemoterapia, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Disciplina Ginecol & Obstet, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Disciplina Hematol & Hemoterapia, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Disciplina Ginecol & Obstet, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 05/55.237-9
dc.identifier.doi 10.1016/j.transci.2012.09.004
dc.description.source Web of Science
dc.identifier.wos WOS:000316646900020



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