RHD gene polymorphisms in alloimmunized RhD-negative individuals with high rate of racial admixture

RHD gene polymorphisms in alloimmunized RhD-negative individuals with high rate of racial admixture

Author Martin, Fabio O. Autor UNIFESP Google Scholar
Menezes, Sidneia S. de Google Scholar
Chiba, Akemi K. Autor UNIFESP Google Scholar
Langhi, Dante M. Google Scholar
Nardozza, Luciano M. M. Autor UNIFESP Google Scholar
Chiattone, Carlos S. Google Scholar
Bordin, Jose O. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fac Ciencias Med Santa Casa São Paulo
Abstract Background: the D-negative phenotype is the result of the total RHD gene deletion in almost all Caucasians, but it accounts for only about 20% in Africans and 70% in Asians. in Africans the RHD Psi that is one of the most important causes of the D-negative phenotype. We investigated the RHD polymorphisms in D-negative phenotype mixed Brazilians who have anti-D alloantibody.Study design and methods: Blood samples from 130 individuals previously typed as D-negative were phenotyped again using: (a) two tube reagents (Anti-D blend reagent, Cellular line TH-28, MS-26; and Anti-D polyclonal); (b) one gel test ID-Card for Rh subgroups including C-w and K antigen; and (c) ABO/Rh (Anti-D blend reagent, Cellular line 175-2, LDM3). the method used for RHD screening detected the presence of RHD exon 10 and intron 4. Sequence analysis was performed on PCR products amplified from genomic DNA for all 10 exons RHD gene.Results: We found that 1181130 (90.8%) of D-negative tested individuals had total RHD gene deletion, while 12/130 (9.2%) showed RHD gene polymorphisms. the RHD Psi was found in 10 (7.7%) individuals, one sample (0.77%) hybrid RHD-CE-D-s /RHD Psi, and another (0.77%) weak D type 4.2.Conclusions: the results showed that the RHD gene was present in 9.2% of racially mixed Brazilians who produced usually clinically significant anti-D alloantibodies. Therefore, the data showed that careful attention is necessary for clinicians in applying RhD genotyping to transfusion medicine in populations with high rate of racial admixture. (C) 2012 Elsevier B.V. All rights reserved.
Keywords RHD gene
Polymorphisms
Anti-D alloantibody
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 05/55.237-9
Date 2013-02-01
Published in Transfusion and Apheresis Science. Oxford: Pergamon-Elsevier B.V., v. 48, n. 1, p. 113-116, 2013.
ISSN 1473-0502 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 113-116
Origin http://dx.doi.org/10.1016/j.transci.2012.09.004
Access rights Closed access
Type Article
Web of Science ID WOS:000316646900020
URI http://repositorio.unifesp.br/handle/11600/35943

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