Suppressive effect of low-level laser therapy on tracheal hyperresponsiveness and lung inflammation in rat subjected to intestinal ischemia and reperfusion

Suppressive effect of low-level laser therapy on tracheal hyperresponsiveness and lung inflammation in rat subjected to intestinal ischemia and reperfusion

Author Lima, Flavia Mafra de Google Scholar
Vitoretti, Luana Google Scholar
Coelho, Fernando Google Scholar
Albertini, Regiane Google Scholar
Breithaupt-Faloppa, Ana Cristina Google Scholar
Lima, Wothan Tavares de Google Scholar
Aimbire, Flavio Autor UNIFESP Google Scholar
Institution Univ Nove Julho
Universidade de São Paulo (USP)
Inst Quim
Universidade Federal de São Paulo (UNIFESP)
Abstract Intestinal ischemia and reperfusion (i-I/R) is an insult associated with acute respiratory distress syndrome (ARDS). It is not known if pro- and anti-inflammatory mediators in ARDS induced by i-I/R can be controlled by low-level laser therapy (LLLT). This study was designed to evaluate the effect of LLLT on tracheal cholinergic reactivity dysfunction and the release of inflammatory mediators from the lung after i-I/R. Anesthetized rats were subjected to superior mesenteric artery occlusion (45 min) and killed after clamp release and preestablished periods of intestinal reperfusion (30 min, 2 or 4 h). the LLLT (660 nm, 7.5 J/cm(2)) was carried out by irradiating the rats on the skin over the right upper bronchus for 15 and 30 min after initiating reperfusion and then euthanizing them 30 min, 2, or 4 h later. Lung edema was measured by the Evans blue extravasation technique, and pulmonary neutrophils were determined by myeloperoxidase (MPO) activity. Pulmonary tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10), intercellular adhesion molecule-1 (ICAM-1), and isoform of NO synthase (iNOS) mRNA expression were analyzed by real-time PCR. TNF-alpha, IL-10, and iNOS proteins in the lung were measured by the enzyme-linked immunoassay technique. LLLT (660 nm, 7.5 J/cm(2)) restored the tracheal hyperresponsiveness and hyporesponsiveness in all the periods after intestinal reperfusion. Although LLLT reduced edema and MPO activity, it did not do so in all the postreperfusion periods. It was also observed with the ICAM-1 expression. in addition to reducing both TNF-alpha and iNOS, LLLT increased IL-10 in the lungs of animals subjected to i-I/R. the results indicate that LLLT can control the lung's inflammatory response and the airway reactivity dysfunction by simultaneously reducing both TNF-alpha and iNOS.
Keywords Gut ischemia
Acute lung inflammation
Airway smooth muscle
Adhesion molecule
Cytokines
Phototherapy
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2008/08048-4
FAPESP: 2008/08838-5
Date 2013-02-01
Published in Lasers in Medical Science. London: Springer London Ltd, v. 28, n. 2, p. 551-564, 2013.
ISSN 0268-8921 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 551-564
Origin http://dx.doi.org/10.1007/s10103-012-1088-1
Access rights Closed access
Type Article
Web of Science ID WOS:000315579400027
URI http://repositorio.unifesp.br/handle/11600/35914

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account