Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis

Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis

Author Hartleben, Claudia P. Google Scholar
Leal, Fernanda M. A. Google Scholar
Monte, Leonardo G. Google Scholar
Hartwig, Daiane D. Google Scholar
Seixas, Fabiana K. Google Scholar
Vasconcellos, Silvio A. Autor UNIFESP Google Scholar
Brihuega, Bibiana Google Scholar
Dellagostin, Odir A. Google Scholar
Institution Univ Fed Pelotas
Universidade Federal de São Paulo (UNIFESP)
INTA
Abstract Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. the signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14-60 days after infection. the reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. in this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. the rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys.
Language English
Sponsor FAPERGS Programa Pesquisador Gaucho-Edital PqG
Grant number FAPERGS Programa Pesquisador Gaucho-Edital PqG: 06/2010
FAPERGS Programa Pesquisador Gaucho-Edital PqG: 1010724
Date 2013-02-01
Published in Current Microbiology. New York: Springer, v. 66, n. 2, p. 106-109, 2013.
ISSN 0343-8651 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 106-109
Origin http://dx.doi.org/10.1007/s00284-012-0237-x
Access rights Closed access
Type Article
Web of Science ID WOS:000313518400002
URI http://repositorio.unifesp.br/handle/11600/35910

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account