Morphometric, quantitative, and three-dimensional analysis of the heart muscle fibers of old rats: Transmission electron microscopy and high-resolution scanning electron microscopy methods

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dc.contributor.author Cury, Diego Pulzatto
dc.contributor.author Dias, Fernando Jose
dc.contributor.author Kronka Sosthenes, Marcia Consentino
dc.contributor.author Dos Santos Haemmerle, Carlos Alexandre
dc.contributor.author Ogawa, Koichi
dc.contributor.author Pereira Da Silva, Marcelo Cavenaghi [UNIFESP]
dc.contributor.author Mardegan Issa, Joao Paulo
dc.contributor.author Iyomasa, Mamie Mizusaki
dc.contributor.author Watanabe, Ii-Sei
dc.date.accessioned 2016-01-24T14:31:10Z
dc.date.available 2016-01-24T14:31:10Z
dc.date.issued 2013-02-01
dc.identifier http://dx.doi.org/10.1002/jemt.22151
dc.identifier.citation Microscopy Research and Technique. Hoboken: Wiley-Blackwell, v. 76, n. 2, p. 184-195, 2013.
dc.identifier.issn 1059-910X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35908
dc.description.abstract This research investigated the morphological, morphometric, and ultrastructural cardiomyocyte characteristics of male Wistar rats at 18 months of age. the animals were euthanized using an overdose of anesthesia (ketamine and xylazine, 150/10 mg/kg) and perfused transcardially, after which samples were collected for light microscopy, transmission electron microscopy, and high-resolution scanning electron microscopy. the results showed that cardiomyocyte arrangement was disposed parallel between the mitochondria and the A-, I-, and H-bands and their M- and Z-lines from the sarcomere. the sarcomere junction areas had intercalated disks, a specific structure of heart muscle. the ultrastructural analysis revealed several mitochondria of various sizes and shapes intermingled between the blood capillaries and their endothelial cells; some red cells inside vessels are noted. the muscle cell sarcolemma could be observed associated with the described structures. the cardiomyocytes of old rats presented an average sarcomere length of 2.071 +/- 0.09 mu m, a mitochondrial volume density (Vv) of 0.3383, a mitochondrial average area of 0.537 +/- 0.278 mu m2, a mitochondrial average length of 1.024 +/- 0.352 mu m, an average mitochondrial cristae thickness of 0.038 +/- 0.09 mu m and a ratio of mitochondrial greater length/lesser length of 1.929 +/- 0.965. of the observed mitochondrial shapes, 23.4% were rounded, 45.3% were elongated, and 31.1% had irregular profiles. in this study, we analyzed the morphology and morphometry of cardiomyocytes in old rats, focusing on mitochondria. These data are important for researchers who focus the changes in cardiac tissue, especially changes owing to pathologies and drug administration that may or may not be correlated with aging. Microsc. Res. Tech., 2013. (C) 2012 Wiley Periodicals, Inc. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship The Faculty of Medicine, Fukuoka University
dc.format.extent 184-195
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Microscopy Research and Technique
dc.rights Acesso restrito
dc.subject heart en
dc.subject old rats en
dc.subject TEM en
dc.subject HRSEM en
dc.subject mitochondria en
dc.title Morphometric, quantitative, and three-dimensional analysis of the heart muscle fibers of old rats: Transmission electron microscopy and high-resolution scanning electron microscopy methods en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Fed Univ Para
dc.contributor.institution Fukuoka Univ
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Dept Surg, Fac Vet Med & Anim Sci, BR-05508900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Anat, Inst Biomed Sci, BR-05508900 São Paulo, Brazil
dc.description.affiliation Fed Univ Para, Inst Biol Sci, Lab Invest Neurodegenerat & Infect, Univ Hosp Joao de Barros Barreto, BR-66059 Belem, Para, Brazil
dc.description.affiliation Fukuoka Univ, Sch Med, Dept Anat, Fukuoka 81401, Japan
dc.description.affiliation Universidade Federal de São Paulo, Dept Morphol & Genet, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Dent, Dept Morphol Stomatol & Physiol, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Morphol & Genet, São Paulo, Brazil
dc.identifier.doi 10.1002/jemt.22151
dc.description.source Web of Science
dc.identifier.wos WOS:000314172900010



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