Morphometric, quantitative, and three-dimensional analysis of the heart muscle fibers of old rats: Transmission electron microscopy and high-resolution scanning electron microscopy methods

Morphometric, quantitative, and three-dimensional analysis of the heart muscle fibers of old rats: Transmission electron microscopy and high-resolution scanning electron microscopy methods

Author Cury, Diego Pulzatto Google Scholar
Dias, Fernando Jose Google Scholar
Kronka Sosthenes, Marcia Consentino Google Scholar
Dos Santos Haemmerle, Carlos Alexandre Google Scholar
Ogawa, Koichi Google Scholar
Pereira Da Silva, Marcelo Cavenaghi Autor UNIFESP Google Scholar
Mardegan Issa, Joao Paulo Google Scholar
Iyomasa, Mamie Mizusaki Google Scholar
Watanabe, Ii-Sei Google Scholar
Institution Universidade de São Paulo (USP)
Fed Univ Para
Fukuoka Univ
Universidade Federal de São Paulo (UNIFESP)
Abstract This research investigated the morphological, morphometric, and ultrastructural cardiomyocyte characteristics of male Wistar rats at 18 months of age. the animals were euthanized using an overdose of anesthesia (ketamine and xylazine, 150/10 mg/kg) and perfused transcardially, after which samples were collected for light microscopy, transmission electron microscopy, and high-resolution scanning electron microscopy. the results showed that cardiomyocyte arrangement was disposed parallel between the mitochondria and the A-, I-, and H-bands and their M- and Z-lines from the sarcomere. the sarcomere junction areas had intercalated disks, a specific structure of heart muscle. the ultrastructural analysis revealed several mitochondria of various sizes and shapes intermingled between the blood capillaries and their endothelial cells; some red cells inside vessels are noted. the muscle cell sarcolemma could be observed associated with the described structures. the cardiomyocytes of old rats presented an average sarcomere length of 2.071 +/- 0.09 mu m, a mitochondrial volume density (Vv) of 0.3383, a mitochondrial average area of 0.537 +/- 0.278 mu m2, a mitochondrial average length of 1.024 +/- 0.352 mu m, an average mitochondrial cristae thickness of 0.038 +/- 0.09 mu m and a ratio of mitochondrial greater length/lesser length of 1.929 +/- 0.965. of the observed mitochondrial shapes, 23.4% were rounded, 45.3% were elongated, and 31.1% had irregular profiles. in this study, we analyzed the morphology and morphometry of cardiomyocytes in old rats, focusing on mitochondria. These data are important for researchers who focus the changes in cardiac tissue, especially changes owing to pathologies and drug administration that may or may not be correlated with aging. Microsc. Res. Tech., 2013. (C) 2012 Wiley Periodicals, Inc.
Keywords heart
old rats
TEM
HRSEM
mitochondria
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
The Faculty of Medicine, Fukuoka University
Date 2013-02-01
Published in Microscopy Research and Technique. Hoboken: Wiley-Blackwell, v. 76, n. 2, p. 184-195, 2013.
ISSN 1059-910X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 184-195
Origin http://dx.doi.org/10.1002/jemt.22151
Access rights Closed access
Type Article
Web of Science ID WOS:000314172900010
URI http://repositorio.unifesp.br/handle/11600/35908

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