A Nature-Inspired Betalainic Probe for Live-Cell Imaging of Plasmodium-Infected Erythrocytes

A Nature-Inspired Betalainic Probe for Live-Cell Imaging of Plasmodium-Infected Erythrocytes

Author Pires Goncalves, Leticia Christina Google Scholar
Tonelli, Renata Rosito Autor UNIFESP Google Scholar
Bagnaresi, Piero Autor UNIFESP Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Ferreira, Antonio Gilberto Google Scholar
Bastos, Erick Leite Google Scholar
Institution Universidade Federal do ABC (UFABC)
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de São Carlos (UFSCar)
Abstract A model betalainic dye was semisynthesized from betanin, the magenta pigment of the red beet, and was effective for live-cell imaging of Plasmodium-infected red blood cells. This water-soluble fluorescent probe is photostable, excitable in the visible region and cell membrane-permeable, and its photophysical properties are not notably pH-sensitive. Fluorescence imaging microscopy of erythrocytes infected with Plasmodium falciparum, a causative agent of malaria in humans, showed that only the parasite was stained. Z-stacking analysis suggested that the probe accumulates proximal to the nucleus of the parasite. Indicaxanthin, one of the natural fluorescent betalains found in the petals of certain flowers, did not stain the parasite or the red blood cell.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordination for the Improvement of Higher Education Personnel
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 07/00684-6
FAPESP: 10/15042-2
FAPESP: 11/23036-5
FAPESP: 07/59407-1
Coordination for the Improvement of Higher Education Personnel: PNPD 427-10/2009
Date 2013-01-16
Published in Plos One. San Francisco: Public Library Science, v. 8, n. 1, 7 p., 2013.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 7
Origin http://dx.doi.org/10.1371/journal.pone.0053874
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000313682700060
URI http://repositorio.unifesp.br/handle/11600/35881

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