Postnatal Transplantation of Interneuronal Precursor Cells Decreases Anxiety-Like Behavior in Adult Mice

Postnatal Transplantation of Interneuronal Precursor Cells Decreases Anxiety-Like Behavior in Adult Mice

Author Valente, Maria Fernanda Autor UNIFESP Google Scholar
Romariz, Simone Amaro Alves Autor UNIFESP Google Scholar
Calcagnotto, Maria Elisa Google Scholar
Ruiz, L. Autor UNIFESP Google Scholar
Mello, Luiz Eugenio Araujo de Moraes Autor UNIFESP Google Scholar
Frussa-Filho, Roberto Autor UNIFESP Google Scholar
Longo, Beatriz M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande do Sul
Abstract The GABAergic system is critically involved in the modulation of anxiety levels, and dysfunction of GABAergic neurotransmission appears to be involved in the development of generalized anxiety disorder. Precursor cells from the medial ganglionic eminence (MGE) have the ability to migrate and differentiate into inhibitory GABAergic interneurons after being transplanted into the mouse brain. Thus, transplantation of interneuronal precursor cells derived from the MGE into a postnatal brain could modify the neuronal circuitry, increasing GABAergic tone and decreasing anxiety-like behavior in animals. Our aim was to verify the in vivo effects of transplanted MGE cells by evaluating anxiety-like behavior in mice. MGE cells from 14-day green fluorescent protein (GFP) embryos were transplanted into newborn mice. At 15, 30, and 60 days posttransplant, the animals were tested for anxiety behavior with the elevated plus maze (EPM) test. Our results show that transplanted cells from MGE were able to migrate to different regions of the brain parenchyma and to differentiate into inhibitory interneurons. the neuronal precursor cell transplanted animals had decreased levels of anxiety, indicating a specific function of these cells in vivo. We suggested that transplantation of MGE-derived neuronal precursors into neonate brain could strengthen the inhibitory function of the GABAergic neuronal circuitry related to anxiety-like behavior in mice.
Keywords GABAergic neuron (GABA)
Medial ganglionic eminence (MGE)
Green fluorescent protein (GFP)
Cell differentiation
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-01-01
Published in Cell Transplantation. Putnam Valley: Cognizant Communication Corp, v. 22, n. 7, p. 1237-1247, 2013.
ISSN 0963-6897 (Sherpa/Romeo, impact factor)
Publisher Cognizant Communication Corp
Extent 1237-1247
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000322501300012

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