Altered of apoptotic markers of both extrinsic and intrinsic pathways induced by hepatitis C virus infection in peripheral blood mononuclear cells

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dc.contributor.author Albertoni, Guilherme [UNIFESP]
dc.contributor.author Arnoni, Carine Prisco
dc.contributor.author Latini, Flavia Roche Moreira
dc.contributor.author Andrade, Sheila Siqueira [UNIFESP]
dc.contributor.author Araujo, Patricia Regina Barboza
dc.contributor.author Rodrigues, Flaviane Kesia
dc.contributor.author Rozenchan, Patricia Bortman [UNIFESP]
dc.contributor.author Mendes-Correa, Maria Cassia
dc.contributor.author Leite, Olavo Henrique Munhoz
dc.contributor.author Schor, Nestor [UNIFESP]
dc.contributor.author Girão, Manoel João Batista Castello [UNIFESP]
dc.contributor.author Barreto, Jose Augusto
dc.date.accessioned 2016-01-24T14:28:09Z
dc.date.available 2016-01-24T14:28:09Z
dc.date.issued 2012-12-20
dc.identifier http://dx.doi.org/10.1186/1743-422X-9-314
dc.identifier.citation Virology Journal. London: Biomed Central Ltd, v. 9, 8 p., 2012.
dc.identifier.issn 1743-422X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35622
dc.description.abstract Background: Chronic hepatitis C (CHC) has emerged as a leading cause of cirrhosis in the U. S. and across the world. To understand the role of apoptotic pathways in hepatitis C virus (HCV) infection, we studied the mRNA and protein expression patterns of apoptosis-related genes in peripheral blood mononuclear cells (PBMC) obtained from patients with HCV infection.Methods: the present study included 50 subjects which plasma samples were positive for HCV, but negative for human immunodeficiency virus (HIV) or hepatitis B virus (HBV). These cases were divided into four groups according to METAVIR, a score-based analysis which helps to interpret a liver biopsy according to the degree of inflammation and fibrosis. mRNA expression of the studied genes were analyzed by reverse transcription of quantitative polymerase chain reaction (RT-qPCR) and protein levels, analyzed by ELISA, was also conducted. HCV genotyping was also determined.Results: HCV infection increased mRNA expression and protein synthesis of caspase 8 in group 1 by 3 fold and 4 fold, respectively (p < 0.05). in group 4 HCV infection increased mRNA expression and protein synthesis of caspase 9 by 2 fold and 1,5 fold, respectively (p < 0.05). Also, caspase 3 mRNA expression and protein synthesis had level augumented by HCV infection in group 1 by 4 fold and 5 fold, respectively, and in group 4 by 6 fold and 7 fold, respectively (p < 0.05).Conclusions: HCV induces alteration at both genomic and protein levels of apoptosis markers involved with extrinsic and intrinsic pathways. en
dc.description.sponsorship Associacao Beneficente de Coleta de Sangue (COLSAN)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Financiadora de Estudos e Projetos (FINEP)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 8
dc.language.iso eng
dc.publisher Biomed Central Ltd
dc.relation.ispartof Virology Journal
dc.rights Acesso aberto
dc.subject HCV infection en
dc.subject Apoptosis en
dc.subject Caspase 3 en
dc.subject Caspase 8 en
dc.subject Caspase 9 en
dc.title Altered of apoptotic markers of both extrinsic and intrinsic pathways induced by hepatitis C virus infection in peripheral blood mononuclear cells en
dc.type Artigo
dc.contributor.institution Colsan Assoc Beneficente Coleta Sangue
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution URDIP
dc.description.affiliation Colsan Assoc Beneficente Coleta Sangue, São Paulo, Brazil
dc.description.affiliation Fed Univ São Paulo UNIFESP, Dept Gynecol, São Paulo, Brazil
dc.description.affiliation URDIP, São Paulo, Brazil
dc.description.affiliation Fed Univ São Paulo UNIFESP, Dept Nephrol, São Paulo, Brazil
dc.description.affiliationUnifesp Fed Univ São Paulo UNIFESP, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUnifesp Fed Univ São Paulo UNIFESP, Dept Nephrol, São Paulo, Brazil
dc.identifier.file WOS000314314500001.pdf
dc.identifier.doi 10.1186/1743-422X-9-314
dc.description.source Web of Science
dc.identifier.wos WOS:000314314500001



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