Altered of apoptotic markers of both extrinsic and intrinsic pathways induced by hepatitis C virus infection in peripheral blood mononuclear cells

Altered of apoptotic markers of both extrinsic and intrinsic pathways induced by hepatitis C virus infection in peripheral blood mononuclear cells

Author Albertoni, Guilherme Autor UNIFESP Google Scholar
Arnoni, Carine Prisco Google Scholar
Latini, Flavia Roche Moreira Google Scholar
Andrade, Sheila Siqueira Autor UNIFESP Google Scholar
Araujo, Patricia Regina Barboza Google Scholar
Rodrigues, Flaviane Kesia Google Scholar
Rozenchan, Patricia Bortman Autor UNIFESP Google Scholar
Mendes-Correa, Maria Cassia Google Scholar
Leite, Olavo Henrique Munhoz Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Girão, Manoel João Batista Castello Autor UNIFESP Google Scholar
Barreto, Jose Augusto Google Scholar
Institution Colsan Assoc Beneficente Coleta Sangue
Universidade Federal de São Paulo (UNIFESP)
URDIP
Abstract Background: Chronic hepatitis C (CHC) has emerged as a leading cause of cirrhosis in the U. S. and across the world. To understand the role of apoptotic pathways in hepatitis C virus (HCV) infection, we studied the mRNA and protein expression patterns of apoptosis-related genes in peripheral blood mononuclear cells (PBMC) obtained from patients with HCV infection.Methods: the present study included 50 subjects which plasma samples were positive for HCV, but negative for human immunodeficiency virus (HIV) or hepatitis B virus (HBV). These cases were divided into four groups according to METAVIR, a score-based analysis which helps to interpret a liver biopsy according to the degree of inflammation and fibrosis. mRNA expression of the studied genes were analyzed by reverse transcription of quantitative polymerase chain reaction (RT-qPCR) and protein levels, analyzed by ELISA, was also conducted. HCV genotyping was also determined.Results: HCV infection increased mRNA expression and protein synthesis of caspase 8 in group 1 by 3 fold and 4 fold, respectively (p < 0.05). in group 4 HCV infection increased mRNA expression and protein synthesis of caspase 9 by 2 fold and 1,5 fold, respectively (p < 0.05). Also, caspase 3 mRNA expression and protein synthesis had level augumented by HCV infection in group 1 by 4 fold and 5 fold, respectively, and in group 4 by 6 fold and 7 fold, respectively (p < 0.05).Conclusions: HCV induces alteration at both genomic and protein levels of apoptosis markers involved with extrinsic and intrinsic pathways.
Keywords HCV infection
Apoptosis
Caspase 3
Caspase 8
Caspase 9
Language English
Sponsor Associacao Beneficente de Coleta de Sangue (COLSAN)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Financiadora de Estudos e Projetos (FINEP)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-12-20
Published in Virology Journal. London: Biomed Central Ltd, v. 9, 8 p., 2012.
ISSN 1743-422X (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 8
Origin http://dx.doi.org/10.1186/1743-422X-9-314
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000314314500001
URI http://repositorio.unifesp.br/handle/11600/35622

Show full item record




File

Name: WOS000314314500001.pdf
Size: 270.7Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account