1,4-Diamino-2-butanone, a putrescine analogue, promotes redox imbalance in Trypanosoma cruzi and mammalian cells

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dc.contributor.author Soares, Chrislaine O
dc.contributor.author Colli, Walter
dc.contributor.author Bechara, Etelvino José Henriques [UNIFESP]
dc.contributor.author Alves, Maria Julia Manso
dc.date.accessioned 2016-01-24T14:28:09Z
dc.date.available 2016-01-24T14:28:09Z
dc.date.issued 2012-12-15
dc.identifier http://dx.doi.org/10.1016/j.abb.2012.09.005
dc.identifier.citation Archives of Biochemistry and Biophysics. New York: Elsevier B.V., v. 528, n. 2, p. 103-110, 2012.
dc.identifier.issn 0003-9861
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35614
dc.description.abstract The putrescine analogue 1,4-diamino-2-butanone (DAB) is highly toxic to various microorganisms, including Trypanosoma cruzi. Similar to other a-aminocarbonyl metabolites. DAB exhibits pro-oxidant properties. DAB undergoes metal-catalyzed oxidation yielding H2O2, NH4+ ion, and a highly toxic alpha-oxoaldehyde. in vitro. DAB decreases mammalian cell viability associated with changes in redox balance. Here, we aim to clarify the DAB pro-oxidant effects on trypomastigotes and on intracellular T. cruzi amastigotes. DAB (0.05-5 mM) exposure in trypomastigotes, the infective stage of T. cruzi, leads to a decline in parasite viability (IC50 c.a. 0.2 mM DAB; 4 h incubation), changes in morphology, thiol redox imbalance, and increased TcSOD activity. Medium supplementation with catalase (2.5 mu M) protects trypomastigotes against DAB toxicity, while host cell invasion by trypomastigotes is hampered by DAB. Additionally, intracellular amastigotes are susceptible to DAB toxicity. Furthermore, pre-treatment with 100-500 mu M buthionine sulfoximine (BSO) of LLC-MK2 potentiates DAB cytotoxicity, whereas 5 mM N-acetyl-cysteine (NAC) protects cells from oxidative stress. Together, these data support the hypothesis that redox imbalance contributes to DAB cytotoxicity in both T. cruzi and mammalian host cells. (C) 2012 Elsevier Inc. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship INCT Processos Redox em Biomedicina - Redoxoma
dc.format.extent 103-110
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Archives of Biochemistry and Biophysics
dc.rights Acesso aberto
dc.subject 1,4-Diamino-2-butanone en
dc.subject Polyamine en
dc.subject alpha-Oxoaldehyde en
dc.subject Trypanosoma cruzi en
dc.subject LLC-MK2 cell en
dc.subject Redox imbalance en
dc.title 1,4-Diamino-2-butanone, a putrescine analogue, promotes redox imbalance in Trypanosoma cruzi and mammalian cells en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Inst Quim, Dept Bioquim, BR-01498 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, Diadema, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, Diadema, SP, Brazil
dc.identifier.file WOS000312236700001.pdf
dc.identifier.doi 10.1016/j.abb.2012.09.005
dc.description.source Web of Science
dc.identifier.wos WOS:000312236700001



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