ANKHD1 regulates cell cycle progression and proliferation in multiple myeloma cells

ANKHD1 regulates cell cycle progression and proliferation in multiple myeloma cells

Author Dhyani, Anamika Google Scholar
Duarte, Adriana S. S. Google Scholar
Machado-Neto, Joao A. Google Scholar
Favaro, Patricia Autor UNIFESP Google Scholar
Ortega, Manoela Marques Google Scholar
Saad, Sara T. Olalla Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Abstract ANKHD1 is a multiple ankyrin repeat containing protein, highly expressed in cancers, such as acute leukemia. the present study was undertaken to determine the expression and functional significance of ANKHD1 in human Multiple Myeloma (MM). We found that ANKHD1 is highly expressed in MM patient cells and cell lines. in vitro, lentiviral mediated ANKHD1-shRNA inhibited proliferation and delayed S to G2M cell cycle progression in glucocorticoid resistant (U266) and sensitive (MM1S) MM cells. Further ANKHD1 silencing resulted in upregulation of cyclin dependent kinase inhibitor p21 irrespective of the p53 status of the MM cell lines. These data suggest that ANKHD1 might have a role in MM cell proliferation and cell cycle progression by regulating expression of p21. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Keywords ANKHD1
Multiple myeloma
Proliferation
Cell cycle
p21
Language English
Sponsor Instituto Nacional de Ciencia e Tecnologia do Sangue(INCTS)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2012-12-14
Published in Febs Letters. Amsterdam: Elsevier B.V., v. 586, n. 24, p. 4311-4318, 2012.
ISSN 0014-5793 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 4311-4318
Origin http://dx.doi.org/10.1016/j.febslet.2012.10.037
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000312004400010
URI http://repositorio.unifesp.br/handle/11600/35612

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