Sesquiterpene lactones and the diterpene 5-epi-icetexone affect the intracellular and extracellular stages of Trypanosoma cruzi

Sesquiterpene lactones and the diterpene 5-epi-icetexone affect the intracellular and extracellular stages of Trypanosoma cruzi

Author Lozano, Esteban Google Scholar
Barrera, Patricia Google Scholar
Salinas, Romina Google Scholar
Vega, Israel Google Scholar
Nieto, Matias Google Scholar
Tonn, Carlos Google Scholar
Kemmerling, Ulrike Google Scholar
Mortara, Renato A. Autor UNIFESP Google Scholar
Sosa, Miguel A. Google Scholar
Institution Univ Cuyo
Univ Nacl San Luis
Univ Chile
Universidade Federal de São Paulo (UNIFESP)
Univ Nacl Cuyo
Abstract Chagas disease is a major health problem in Latin America and is caused by the parasitic protozoan Trypanosoma cruzi. Although many drugs have been used to alleviate the disease, these have been ineffective in the chronic phase and have also presented numerous side effects on patients. in this study we tested the effect of three sesquiterpene lactones (dehydroleucodine, helenalin and mexicanin) and a diterpene (5-epi-icetexone) on parasites (Y-strain) grown in host cells. At 48 h of treatment, the number of amastigotes inside the cells was lower than in the controls. This effect was observable at concentrations of 1.5-3.8 mu M, which are of low cytotoxicity to host cells. in addition, the compounds caused a decrease in the percentage of infected cells. the treatments also reduced the presence of trypomastigotes in the extracellular medium. in all cases, helenalin was the most potent. the number of parasites per cell at 24 h indicates the occurrence of multiple infection, which would also be affected by the compounds. However, we should not discard an effect on the proliferation and survival of parasites within the host cells. On the other hand, an additional effect on the differentiation of parasites and/or the survival of extracellular trypomastigotes might be possible. We conclude that these compounds are very effective against T cruzi possibly by multiple mechanisms. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
Keywords Chagas disease
Trypanosoma cruzi
Natural compounds
Sesquiterpene lactones
Diterpenes
Language English
Sponsor University of Cuyo (SECyT)
AMSUD/Pasteur
Grant number University of Cuyo (SECyT): 06/J280
Date 2012-12-01
Published in Parasitology International. Clare: Elsevier B.V., v. 61, n. 4, p. 628-633, 2012.
ISSN 1383-5769 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 628-633
Origin http://dx.doi.org/10.1016/j.parint.2012.06.005
Access rights Closed access
Type Article
Web of Science ID WOS:000309376800018
URI http://repositorio.unifesp.br/handle/11600/35572

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