Glycosaminoglycan profiles in the uterus of adult ovariectomized rats treated with estrogen and progestagen

Glycosaminoglycan profiles in the uterus of adult ovariectomized rats treated with estrogen and progestagen

Author Simoes, Ricardo Santos Google Scholar
Oliveira-Filho, Ricardo Martins Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Baracat, Edmund Chada Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Objectives: To evaluate the effects of conjugated equine estrogens (CE) alone or in combination with medroxyprogesterone acetate (MPA) on glycosaminoglycans (GAGs) in the cervix and horns of the rat uterus.Study design: Thirty days after ovariectomy, adult rats were randomly divided into four groups: Cl, control (treated with drug vehicle); GII CE (50 mu g/kg per day); GIII, MPA (0.2 mg/kg per day), and GIV, CE + MPA (doses as in GII and Gill). Drugs and vehicle were given by gavage during 28 days. Afterwards the animals were anesthetized, the cervix and uterine horns were dissected out and the middle portion fixed in 10% formaldehyde solution; other portions were fixed in acetone for histological examination and glycosaminoglycan quantification, respectively. Agarose gel electrophoresis was used for sulfated GAG analyses, and hyaluronic acid was assayed with an ELISA-like method. Statistical analysis was done by the Student's t test and the Tukey-Kramer test (P < 0.05).Results: the cervix and uterine horn structures presented signs of atrophy in the control group (GI). the other groups, mainly groups III and IV, had histological aspects of proliferation. in all groups the concentration of sulfated GAGs (especially dermatan sulfate) was higher than that of non-sulfated GAGs, both in cervix and in uterine horns. Estrogens increased sulfated GAG concentration at the cervix and the horn, whereas in uterine horns the amounts of sulfated GAGs were decreased after estrogens plus MPA treatment. the concentration of hyaluronic acid in uterine horns was higher than in cervices.Conclusions: the profiling and amounts of glycosaminoglycans in the two portions of the rat uterus are uneven. Dermatan sulfate occurs in higher concentrations in both cervix and uterine horns. Sulfated GAGs in rat cervix were increased by estrogens plus MPA, but were decreased by MPA alone in uterine horns. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
Keywords Estrogens
Conjugated (USP)
Medroxyprogesterone acetate
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number CNPq: 302966/2008
Date 2012-12-01
Published in European Journal of Obstetrics & Gynecology and Reproductive Biology. Amsterdam: Elsevier B.V., v. 165, n. 2, p. 265-270, 2012.
ISSN 0301-2115 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 265-270
Access rights Closed access
Type Article
Web of Science ID WOS:000313317300022

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