Heterogeneous time-dependent response of adipose tissue during the development of cancer cachexia

Heterogeneous time-dependent response of adipose tissue during the development of cancer cachexia

Author Batista, M. L. Google Scholar
Neves, R. X. Google Scholar
Peres, S. B. Google Scholar
Yamashita, A. S. Google Scholar
Shida, C. S. Autor UNIFESP Google Scholar
Farmer, S. R. Google Scholar
Seelaender, M. Google Scholar
Institution Univ Mogi Das Cruzes
Universidade Estadual de Maringá (UEM)
Universidade de São Paulo (USP)
Boston Univ
Universidade Federal de São Paulo (UNIFESP)
Abstract Cancer cachexia induces loss of fat mass that accounts for a large part of the dramatic weight loss observed both in humans and in animal models; however, the literature does not provide consistent information regarding the set point of weight loss and how the different visceral adipose tissue depots contribute to this symptom. To evaluate that, 8-week-old male Wistar rats were subcutaneously inoculated with 1 ml (2 x 10(7)) of tumour cells (Walker 256). Samples of different visceral white adipose tissue (WAT) depots were collected at days 0, 4, 7 and 14 and stored at -80 degrees C (seven to ten animals/each day per group). Mesenteric and retroperitoneal depot mass was decreased to the greatest extent on day 14 compared with day 0. Gene and protein expression of PPAR gamma(2) (PPARG) fell significantly following tumour implantation in all three adipose tissue depots while C/EBP alpha (CEBPA) and SREBP-1c (SREBF1) expression decreased over time only in epididymal and retroperitoneal depots. Decreased adipogenic gene expression and morphological disruption of visceral WAT are further supported by the dramatic reduction in mRNA and protein levels of perilipin. Classical markers of inflammation and macrophage infiltration (f4/80, CD68 and MIF-1 alpha) in WAT were significantly increased in the later stage of cachexia (although showing a incremental pattern along the course of cachexia) and presented a depot-specific regulation. These results indicate that impairment in the lipid-storing function of adipose tissue occurs at different times and that the mesenteric adipose tissue is more resistant to the 'fat-reducing effect' than the other visceral depots during cancer cachexia progression. Journal of Endocrinology (2012) 215, 363-373
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2007/52782-1
FAPESP: 2010/51078-1
Date 2012-12-01
Published in Journal of Endocrinology. Bristol: Bioscientifica Ltd, v. 215, n. 3, p. 363-373, 2012.
ISSN 0022-0795 (Sherpa/Romeo, impact factor)
Publisher Bioscientifica Ltd
Extent 363-373
Origin http://dx.doi.org/10.1530/JOE-12-0307
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000310881600004
URI http://repositorio.unifesp.br/handle/11600/35532

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