Evaluation of the Susceptibility profiles, genetic similarity and presence of qnr gene in Escherichia coli resistant to ciprofloxacin isolated in Brazilian hospitals

Evaluation of the Susceptibility profiles, genetic similarity and presence of qnr gene in Escherichia coli resistant to ciprofloxacin isolated in Brazilian hospitals

Autor Pereira, Andrea dos Santos Autor UNIFESP Google Scholar
Andrade, Soraya Sgambatti Autor UNIFESP Google Scholar
Monteiro, Jussimara Autor UNIFESP Google Scholar
Sader, Helio Silva Autor UNIFESP Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
Gales, Ana Cristina Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
JMI Laboratories
Resumo Increasing quinolone resistance has been reported worldwide, mainly among clinical isolates of Escherichia coli. The objectives of this study were to determine the susceptibility profile, the genetic relatedness, and the prevalence of the qnr gene among ciprofloxacin-resistant Escherichia coli isolated from distinct Brazilian hospitals. A total of 144 ciprofloxacin-resistant Escherichia coli were isolated from 17 Brazilian hospitals between January/2002 and June/2003. The antimicrobial susceptibility testing was performed by microdilution according to NCCLS. The presence of the qnr gene was initially screened by colony blotting, and then confirmed by PCR followed by DNA sequencing. Ninety-five urinary ciprofloxacin-resistant Escherichia coli were further selected for molecular typing by pulsed-field gel electrophoresis (PFGE). Imipenem and meropenem showed the highest susceptibility rates (100.0% for both compounds) followed by amikacin (91.0%) and piperacillin/tazobactan (84.8%). A single ciprofloxacin-resistant Escherichia coli isolate was positive for qnr among the 144 ciprofloxacin-resistant Escherichia coli. Forty-six PFGE patterns were observed among the 95 ciprofloxacin-resistant Escherichia coli type. This study shows that therapeutic options are limited for treatment of ciprofloxacin-resistant Escherichia coli due to the presence of additional mechanisms of antimicrobial resistance, such as ESBL production. The qnr gene was uncommon among ciprofloxacin-resistant Escherichia coli clinical isolates, but its identification might indicate the emergence of this mechanism of quinolone resistance in Brazil. The great genomic variability found among the ciprofloxacin-resistant Escherichia coli highlights the importance of the appropriate use of quinolone to restrict the selection of resistant isolates.
Palavra-chave Quinolone resistance
Escherichia coli
PFGE
qnr
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Data de publicação 2007-02-01
Publicado em Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 11, n. 1, p. 40-43, 2007.
ISSN 1413-8670 (Sherpa/Romeo, fator de impacto)
Publicador Brazilian Society of Infectious Diseases
Extensão 40-43
Fonte http://dx.doi.org/10.1590/S1413-86702007000100011
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000253997600011
SciELO S1413-86702007000100011 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/3538

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