Brown spider (Loxosceles intermedia) venom triggers endothelial cells death by anoikis

Brown spider (Loxosceles intermedia) venom triggers endothelial cells death by anoikis

Author Nowatzki, Jenifer Google Scholar
Sene, Reginaldo Vieira Google Scholar
Paludo, Katia Sabrina Google Scholar
Rizzo, Luiz Eduardo Google Scholar
Souza-Fonseca-Guimaraes, Fernando Google Scholar
Veiga, Silvio Sanches Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Franco, Celia Regina C. Google Scholar
Trindade, Edvaldo S. Google Scholar
Institution Univ Fed Parana
Universidade Federal de São Paulo (UNIFESP)
Estadual Univ Parana
Abstract Brown spider (Loxosceles sp.) venom affects the endothelium of vessels and triggers disruptive activity in the subendothelial matrix. the vascular disorders observed after venom exposure include leukocyte and platelet activation, disseminated intravascular coagulation, an increase in vessel permeability and hemorrhage into the dermis. in this study, we report additional evidence regarding the mechanism of endothelial cell cytotoxicity induced by Loxosceles intermedia venom. Exposure to venom led to endothelial cell detachment in a time-dependent manner. Loss of cell anchorage and cell-cell adhesion following venom exposure was accompanied by changes in the distribution of the alpha(5)beta(1) integrin and VE-cadherin. An ultrastructural analysis of cells treated with venom revealed morphological alterations characteristic of apoptosis. Moreover, after venom exposure, the ratio between Bax and Bcl-2 proteins was disturbed in favor of Bax. in addition, late apoptosis was only observed in cells detached by the action of venom. Accordingly, there was no increase in apoptosis when cells were exposed to L. intermedia venom in suspension, suggesting that the loss of cell anchorage provides the signal to initiate apoptosis. Thus. L. intermedia venom likely triggers endothelial cell death indirectly through an apoptotic mechanism known as anoikis. (C) 2012 Elsevier B.V. All rights reserved.
Keywords Loxosceles intermedia
Venom
Anoikis
Apoptosis
Endothelial cells
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundacao Araucaria-PR
Secretaria de Estado de Ciencia, Tecnologia e Ensino Superior do Parana (SETI), Brazil
Date 2012-09-01
Published in Toxicon. Oxford: Pergamon-Elsevier B.V., v. 60, n. 3, p. 396-405, 2012.
ISSN 0041-0101 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 396-405
Origin http://dx.doi.org/10.1016/j.toxicon.2012.04.333
Access rights Closed access
Type Article
Web of Science ID WOS:000306869300020
URI http://repositorio.unifesp.br/handle/11600/35238

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