Early Diagnosis of Prostate Cancer by Citrate Determination in Urine with Europium-Oxytetracycline Complex

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dc.contributor.author Silva, Flavia R. O. [UNIFESP]
dc.contributor.author Nabeshima, Camila Tiemi [UNIFESP]
dc.contributor.author Bellini, Maria H.
dc.contributor.author Courrol, Lilia C. [UNIFESP]
dc.date.accessioned 2016-01-24T14:27:33Z
dc.date.available 2016-01-24T14:27:33Z
dc.date.issued 2012-08-01
dc.identifier http://dx.doi.org/10.1366/11-06546
dc.identifier.citation Applied Spectroscopy. Frederick: Soc Applied Spectroscopy, v. 66, n. 8, p. 958-961, 2012.
dc.identifier.issn 0003-7028
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35161
dc.description.abstract Normal prostate tissue contains high levels of citrate. in the presence of prostate cancer, the citrate level is diminished. in this paper we show that it is possible to use europium oxytetracycline complex as a citrate fluorescent probe and consequently as a prostate cancer probe. We analyzed normal nude male mice urine and urine from nude male mice in which prostate cancer was induced by intraprostatic inoculation of DU145 cells. the urine samples were collected from the animals at the 7th, 14th, 21st, and 35th days after the surgery procedures. the intensity of europium emission at 615 nm in europium oxytetracycline complex in the presence of citrate increases linearly. the citrate concentrations were determined from a calculated calibration curve. A concentration decrease in malignant prostate urine from the normal (PBS group) urine value from similar to 8.0 mM to similar to 2.4 mM (tumor group at 35th day) was found. the obtained results indicated that europium oxytetracycline provides a significant biomarker for prostate cancer detection with a direct, accurate, noninvasive, and non-enzymatic method for measurement of citrate in biological fluids. en
dc.format.extent 958-961
dc.language.iso eng
dc.publisher Soc Applied Spectroscopy
dc.relation.ispartof Applied Spectroscopy
dc.rights Acesso restrito
dc.subject Citrate en
dc.subject Europium en
dc.subject Tetracycline en
dc.subject Fluorescence en
dc.subject Fluorescent probe en
dc.subject Prostate cancer en
dc.title Early Diagnosis of Prostate Cancer by Citrate Determination in Urine with Europium-Oxytetracycline Complex en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution IPEN CNEN SP
dc.description.affiliation Universidade Federal de São Paulo, Dept Nefrol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, Brazil
dc.description.affiliation IPEN CNEN SP, Inst Pesquisas Energet & Nucl, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Nefrol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, Brazil
dc.identifier.doi 10.1366/11-06546
dc.description.source Web of Science
dc.identifier.wos WOS:000306720600013



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