Early Diagnosis of Prostate Cancer by Citrate Determination in Urine with Europium-Oxytetracycline Complex

Early Diagnosis of Prostate Cancer by Citrate Determination in Urine with Europium-Oxytetracycline Complex

Author Silva, Flavia R. O. Autor UNIFESP Google Scholar
Nabeshima, Camila Tiemi Autor UNIFESP Google Scholar
Bellini, Maria H. Google Scholar
Courrol, Lilia C. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
IPEN CNEN SP
Abstract Normal prostate tissue contains high levels of citrate. in the presence of prostate cancer, the citrate level is diminished. in this paper we show that it is possible to use europium oxytetracycline complex as a citrate fluorescent probe and consequently as a prostate cancer probe. We analyzed normal nude male mice urine and urine from nude male mice in which prostate cancer was induced by intraprostatic inoculation of DU145 cells. the urine samples were collected from the animals at the 7th, 14th, 21st, and 35th days after the surgery procedures. the intensity of europium emission at 615 nm in europium oxytetracycline complex in the presence of citrate increases linearly. the citrate concentrations were determined from a calculated calibration curve. A concentration decrease in malignant prostate urine from the normal (PBS group) urine value from similar to 8.0 mM to similar to 2.4 mM (tumor group at 35th day) was found. the obtained results indicated that europium oxytetracycline provides a significant biomarker for prostate cancer detection with a direct, accurate, noninvasive, and non-enzymatic method for measurement of citrate in biological fluids.
Keywords Citrate
Europium
Tetracycline
Fluorescence
Fluorescent probe
Prostate cancer
Language English
Date 2012-08-01
Published in Applied Spectroscopy. Frederick: Soc Applied Spectroscopy, v. 66, n. 8, p. 958-961, 2012.
ISSN 0003-7028 (Sherpa/Romeo, impact factor)
Publisher Soc Applied Spectroscopy
Extent 958-961
Origin http://dx.doi.org/10.1366/11-06546
Access rights Closed access
Type Article
Web of Science ID WOS:000306720600013
URI http://repositorio.unifesp.br/handle/11600/35161

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