Synovial fluid chondroitin sulphate indicates abnormal joint metabolism in asymptomatic osteochondritic horses

Show simple item record

dc.contributor.author Machado, T. S. L.
dc.contributor.author Correia da Silva, L. C. L.
dc.contributor.author Baccarin, Raquel Yvonne Arantes [UNIFESP]
dc.contributor.author Michelacci, Y. M. [UNIFESP]
dc.date.accessioned 2016-01-24T14:27:27Z
dc.date.available 2016-01-24T14:27:27Z
dc.date.issued 2012-07-01
dc.identifier http://dx.doi.org/10.1111/j.2042-3306.2011.00539.x
dc.identifier.citation Equine Veterinary Journal. Hoboken: Wiley-Blackwell, v. 44, n. 4, p. 404-411, 2012.
dc.identifier.issn 0425-1644
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35077
dc.description.abstract Reasons for performing study: Alternative methods to evaluate the joint condition in asymptomatic osteochondrosis dissecans (OCD) and other joint diseases may be useful. Objectives: To investigate possible changes in synovial fluid composition that may lead to joint conditions in asymptomatic OCD, in mature horses. Methods: Animals aged >2 years, of different breeds, with OCD in the intermediate ridge of distal tibia, symptomatic or not, were studied. Synovial fluid samples (10 healthy; 11 asymptomatic OCD; 25 symptomatic OCD) were collected by arthroscopy from 29 horses. Glycosaminoglycans (GAGs) were analysed by a combination of agarose gel electrophoresis and enzymatic degradation with specific GAG lyases. the viscosity, white blood cell (WBC) count, protein concentration and hyaluronic acid (HA) molecular weight were also determined. Results: the method used here to analyse synovial fluid GAGs is reliable, reproducible and specific. the main synovial fluid GAGs are HA and chondroitin sulphate (CS), 93% and 7% respectively in normal horses. in symptomatic OCD, the concentrations of both increased (expressed as GAG/urea ratios), but CS increased more. the CS increased also in asymptomatic OCD. An inflammatory reaction was suggested by the increased WBC counts in OCD. the molecular weight of the synovial fluid HA was reduced in OCD, explaining the lower viscosity observed. Conclusions: the increased CS in synovial fluid of OCD joints in mature horses suggests that the synovial fluid CS and the WBC count are good markers of the joint conditions, allowing the identification of pathological phase in joint diseases. Potential relevance: the analysis of synovial fluid GAGs shows that cartilage damage occurs even in asymptomatic OCD, implying that arthroscopic removal of osteochondral fragments should be performed even in asymptomatic OCD. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Sociedade Paulista para o Desenvolvimento da Medicina (SPDM - FADA), São Paulo, SP, Brazil
dc.description.sponsorship SPDM
dc.format.extent 404-411
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Equine Veterinary Journal
dc.rights Acesso restrito
dc.subject horse en
dc.subject chondroitin sulphate en
dc.subject glycosaminoglycan en
dc.subject hyaluronic acid en
dc.subject osteochondrosis dissecans en
dc.subject synovial fluid en
dc.title Synovial fluid chondroitin sulphate indicates abnormal joint metabolism in asymptomatic osteochondritic horses en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Cirurgia, Fac Med Vet & Zootecnia, BR-09500900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Clin Med, Fac Med Vet & Zootecnia, BR-09500900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, São Paulo, Brazil
dc.identifier.doi 10.1111/j.2042-3306.2011.00539.x
dc.description.source Web of Science
dc.identifier.wos WOS:000304864300008



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account