Use of lutein and zeaxanthin alone or combined with Brilliant Blue to identify intraocular structures intraoperatively

Use of lutein and zeaxanthin alone or combined with Brilliant Blue to identify intraocular structures intraoperatively

Author Sousa-Martins, Diogo Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Moraes, Milton Nunes de Autor UNIFESP Google Scholar
Lima-Filho, Acacio Alves de Souza Autor UNIFESP Google Scholar
Rodrigues, Eduardo Buchele Autor UNIFESP Google Scholar
Chen, Jane Autor UNIFESP Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Santos, Leticia B. Google Scholar
Belfort, Rubens Junior Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Purpose: To determine whether a natural dye solution based on lutein and zeaxanthin alone or combined with Brilliant Blue stains and facilitates peeling of intraocular membranes in human eyes.Methods: in this study of 60 cadaveric eyes, open-sky vitrectomy including posterior hyaloid detachment was performed. Different lutein and zeaxanthin concentrations (0.01-20%) were tested alone or combined with different Brilliant Blue concentrations (0.0125-0.025%) in the corneal endothelium, corneal epithelium, anterior and posterior capsule, vitreous cavity through the macula including the posterior hyaloid, and internal limiting membrane. the various dye solutions were in contact with the intraocular membranes for <1 minute and then were removed by mechanical aspiration or membrane peeling initiated and completed with intraocular forceps. the specimens were examined by light and electron transmission microscopy.Results: Contact between lutein and zeaxanthin and the retinal, lens, and vitreous surface resulted in orange and greenish staining of the intraocular membranes, which facilitated surgical steps in all eyes. Lutein and zeaxanthin alone was useful for vitreous identification and lutein and zeaxanthin combined with Brilliant Blue had strong affinity for internal limiting membrane and anterior capsule. Light microscopy confirmed internal limiting membrane removal in all eyes tested. No dye solutions remained in the eyes after the membrane removal.Conclusion: A natural dye solution based on lutein and zeaxanthin alone or combined with Brilliant Blue efficiently stained the anterior capsule, vitreous, and internal limiting membrane in human cadaveric eyes and may be a useful tool for vitreoretinal or cataract surgery. RETINA 32:1328-1336, 2012
Keywords blue
brilliant
capsule
chromovitrectomy
dye
internal limiting membrane
lutein
safety
vitreous
zeaxanthin
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Kemin Industries, Inc
Ophthalmos Industria e Comercio de Produtos Farmaceuticos S.A.
Date 2012-07-01
Published in Retina-the Journal of Retinal and Vitreous Diseases. Philadelphia: Lippincott Williams & Wilkins, v. 32, n. 7, p. 1328-1336, 2012.
ISSN 0275-004X (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 1328-1336
Origin http://dx.doi.org/10.1097/IAE.0b013e318239e2b6
Access rights Closed access
Type Article
Web of Science ID WOS:000305782100015
URI http://repositorio.unifesp.br/handle/11600/35072

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