The levels of renin-angiotensin related components are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy

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dc.contributor.author Furtado Gouveia, Telma Luciana [UNIFESP]
dc.contributor.author Berzaghi Frangiotti, Maria Isabel [UNIFESP]
dc.contributor.author Vieira de Brito, Joise Marques [UNIFESP]
dc.contributor.author Castro Neto, Eduardo Ferreira de [UNIFESP]
dc.contributor.author Sakata, Maisa Mayume [UNIFESP]
dc.contributor.author Febba, Andreia Cristina [UNIFESP]
dc.contributor.author Casarini, Dulce Elena [UNIFESP]
dc.contributor.author Amado, Debora [UNIFESP]
dc.contributor.author Cavalheiro, Esper Abrao [UNIFESP]
dc.contributor.author Almeida, Sandro Soares [UNIFESP]
dc.contributor.author Manchini, Martha Trindade
dc.contributor.author Araujo, Ronaldo Carvalho [UNIFESP]
dc.contributor.author Silva, Jose Antonio
dc.contributor.author Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
dc.date.accessioned 2016-01-24T14:27:26Z
dc.date.available 2016-01-24T14:27:26Z
dc.date.issued 2012-07-01
dc.identifier http://dx.doi.org/10.1016/j.neuint.2012.04.012
dc.identifier.citation Neurochemistry International. Oxford: Pergamon-Elsevier B.V., v. 61, n. 1, p. 54-62, 2012.
dc.identifier.issn 0197-0186
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35063
dc.description.abstract We previously showed that patients with temporal lobe epilepsy (TLE) present an increased expression of angiotensin II (AngII) AT1 and AT2 receptors in the hippocampus, supporting the idea of an upregulation of renin-angiotensin system (RAS) in this disease.This study aimed to verify the relationship between the RAS and TLE during epileptogenesis. Levels of the peptides angiotensin I (AngI), angiotensin II (Ana) and angiotensin 1-7 (Ang 1-7), were detected by HPLC assay. Angiotensin All and AT2 receptors, Mas mRNA receptors and angiotensin converting enzyme (ACE), tonin and neutral endopeptidase (NEP) mRNA were also quantified at the hippocampus of Wistar rats by real time PCR, during acute (n = 10), silent (n = 10) and chronic (n = 10) phases of pilocarpine-induced epilepsy.We observed an increased peptide level of Ang1-7 into acute and silent phases, decreasing importantly (p <= 0.05) in the chronic phase, suggesting that AngI may be converted into Ang 1-7 by NEP, which is present in high levels in these periods.Our results also showed increased peptide level of AngII in the chronic phase of this model. in contraposition, the ACE expression is reduced in all periods. These data suggest that angiotensinogen or AngI may be cleaved to AngII by tonin, which showed increased expression in all phases. We found changes in AT1, AT2 and Mas mRNA receptors levels suggesting that Ang1-7 could act at Mas receptor during the silent period. Herein, we demonstrated for the first time, changes in angiotensin-related peptides, their receptors as well as the releasing enzymes in the hippocampus of rats during pilocarpine-induced epilepsy. (C) 2012 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Cooperacao Interinstitucional de Apoio a Pesquisa sobre o Cerebro (CInAPCe)
dc.description.sponsorship Instituto Nacional de Neurociencia Translacional (INNT) (Brazil)
dc.format.extent 54-62
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Neurochemistry International
dc.rights Acesso restrito
dc.subject Pilocarpine en
dc.subject Angiotensin I en
dc.subject Angiotensin II en
dc.subject Temporal lobe epilepsy en
dc.subject Angiotensin converting enzyme (ACE) en
dc.title The levels of renin-angiotensin related components are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Nove Julho
dc.description.affiliation Universidade Federal de São Paulo UNIFESP, Dept Biochem, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo UNIFESP, Dept Neurol & Neurosurg, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo UNIFESP, Dept Med, Nephrol Lab, São Paulo, Brazil
dc.description.affiliation Univ Nove Julho, Rehabil Dept, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo UNIFESP, Dept Biophys, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP, Dept Neurol & Neurosurg, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP, Dept Med, Nephrol Lab, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP, Dept Biophys, São Paulo, Brazil
dc.identifier.doi 10.1016/j.neuint.2012.04.012
dc.description.source Web of Science
dc.identifier.wos WOS:000306619300008



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