The levels of renin-angiotensin related components are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy

The levels of renin-angiotensin related components are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy

Author Furtado Gouveia, Telma Luciana Autor UNIFESP Google Scholar
Berzaghi Frangiotti, Maria Isabel Autor UNIFESP Google Scholar
Vieira de Brito, Joise Marques Autor UNIFESP Google Scholar
Castro Neto, Eduardo Ferreira de Autor UNIFESP Google Scholar
Sakata, Maisa Mayume Autor UNIFESP Google Scholar
Febba, Andreia Cristina Autor UNIFESP Google Scholar
Casarini, Dulce Elena Autor UNIFESP Google Scholar
Amado, Debora Autor UNIFESP Google Scholar
Cavalheiro, Esper Abrao Autor UNIFESP Google Scholar
Almeida, Sandro Soares Autor UNIFESP Google Scholar
Manchini, Martha Trindade Google Scholar
Araujo, Ronaldo Carvalho Autor UNIFESP Google Scholar
Silva, Jose Antonio Google Scholar
Naffah-Mazzacoratti, Maria da Graca Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Nove Julho
Abstract We previously showed that patients with temporal lobe epilepsy (TLE) present an increased expression of angiotensin II (AngII) AT1 and AT2 receptors in the hippocampus, supporting the idea of an upregulation of renin-angiotensin system (RAS) in this disease.This study aimed to verify the relationship between the RAS and TLE during epileptogenesis. Levels of the peptides angiotensin I (AngI), angiotensin II (Ana) and angiotensin 1-7 (Ang 1-7), were detected by HPLC assay. Angiotensin All and AT2 receptors, Mas mRNA receptors and angiotensin converting enzyme (ACE), tonin and neutral endopeptidase (NEP) mRNA were also quantified at the hippocampus of Wistar rats by real time PCR, during acute (n = 10), silent (n = 10) and chronic (n = 10) phases of pilocarpine-induced epilepsy.We observed an increased peptide level of Ang1-7 into acute and silent phases, decreasing importantly (p <= 0.05) in the chronic phase, suggesting that AngI may be converted into Ang 1-7 by NEP, which is present in high levels in these periods.Our results also showed increased peptide level of AngII in the chronic phase of this model. in contraposition, the ACE expression is reduced in all periods. These data suggest that angiotensinogen or AngI may be cleaved to AngII by tonin, which showed increased expression in all phases. We found changes in AT1, AT2 and Mas mRNA receptors levels suggesting that Ang1-7 could act at Mas receptor during the silent period. Herein, we demonstrated for the first time, changes in angiotensin-related peptides, their receptors as well as the releasing enzymes in the hippocampus of rats during pilocarpine-induced epilepsy. (C) 2012 Elsevier B.V. All rights reserved.
Keywords Pilocarpine
Angiotensin I
Angiotensin II
Temporal lobe epilepsy
Angiotensin converting enzyme (ACE)
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Cooperacao Interinstitucional de Apoio a Pesquisa sobre o Cerebro (CInAPCe)
Instituto Nacional de Neurociencia Translacional (INNT) (Brazil)
Date 2012-07-01
Published in Neurochemistry International. Oxford: Pergamon-Elsevier B.V., v. 61, n. 1, p. 54-62, 2012.
ISSN 0197-0186 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 54-62
Access rights Closed access
Type Article
Web of Science ID WOS:000306619300008

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