High-fat diets rich in soy or fish oil distinctly alter hypothalamic insulin signaling in rats

High-fat diets rich in soy or fish oil distinctly alter hypothalamic insulin signaling in rats

Author Pimentel, Gustavo D. Autor UNIFESP Google Scholar
Dornellas, Ana P. S. Autor UNIFESP Google Scholar
Rosa, Jose C. Autor UNIFESP Google Scholar
Lira, Fabio S. Autor UNIFESP Google Scholar
Cunha, Claudio A. Autor UNIFESP Google Scholar
Boldarine, Valter T. Autor UNIFESP Google Scholar
Souza, Gabriel I. H. de Autor UNIFESP Google Scholar
Hirata, Aparecida E. Autor UNIFESP Google Scholar
Nascimento, Claudia M. O. Autor UNIFESP Google Scholar
Oyama, Lila M. Autor UNIFESP Google Scholar
Watanabe, Regina L. H. Autor UNIFESP Google Scholar
Ribeiro, Eliane B. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Hypothalamic insulin inhibits food intake, preventing obesity. High-fat feeding with polyunsaturated fats may be obesogenic, but their effect on insulin action has not been elucidated. the present study evaluated insulin hypophagia and hypothalamic signaling after central injection in rats fed either control diet (15% energy from fat) or high-fat diets (50% energy from fat) enriched with either soy or fish oil. Soy rats had increased fat pad weight and serum leptin with normal body weight, serum lipid profile and peripheral insulin sensitivity. Fish rats had decreased body and fat pad weight, low leptin and corticosterone levels, and improved serum lipid profile. A 20-mU dose of intracerebroventricular (ICV) insulin inhibited food intake in control and fish groups, but failed to do so in the soy group. Hypothalamic protein levels of IR. IRS-1, IRS-2, Akt, mTOR, p70S6K and AMPK were similar among groups. ICV insulin stimulated IR tyrosine phosphorylation in control (68%), soy (36%) and fish (34%) groups. Tyrosine phosphorylation of the pp185 band was significantly stimulated in control (78%) and soy (53%) rats, but not in fish rats. IRS-1 phosphorylation was stimulated only in control rats (94%). Akt serine phosphorylation was significantly stimulated only in control (90%) and fish (78%) rats. the results showed that, rather than the energy density, the fat type was a relevant aspect of high-fat feeding, since blockade of hypothalamic insulin signal transmission and insulin hypophagia was promoted only by the high-fat soy diet, while they were preserved in the rats fed with the high-fat fish diet. (C) 2012 Elsevier Inc. All rights reserved.
Keywords Polyunsaturated fatty acids
Hypothalamus
Insulin signaling
Obesity
Insulin hypophagia
Food intake
High-fat feeding
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-07-01
Published in Journal of Nutritional Biochemistry. New York: Elsevier B.V., v. 23, n. 7, p. 822-828, 2012.
ISSN 0955-2863 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 822-828
Origin http://dx.doi.org/10.1016/j.jnutbio.2011.04.006
Access rights Closed access
Type Article
Web of Science ID WOS:000305544000017
URI http://repositorio.unifesp.br/handle/11600/35061

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