Rats With Different Thresholds to Clonic Convulsions Induced by DMCM Differ in the Binding of [H-3]-MK-801 and [H-3]-Ouabain in the Membranes of Brain Regions

Rats With Different Thresholds to Clonic Convulsions Induced by DMCM Differ in the Binding of [H-3]-MK-801 and [H-3]-Ouabain in the Membranes of Brain Regions

Author Contó, Marcos Brandão Autor UNIFESP Google Scholar
Barbosa de Carvalho, Jose Gilberto Autor UNIFESP Google Scholar
Venditti, Marco Antonio Campana Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Considering the putative participation of N-methyl-D-aspartate (NMDA) receptors and the Na+, K+-ATPase enzymes in the susceptibility to convulsions induced by the benzodiazepine inverse agonist methyl 6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate (DMCM), the present study sought to determine if rats with high (HTR) and low (LTR) thresholds to clonic convulsions induced by DMCM differed in the following aspects: the binding of NMDA receptors by [H-3]-MK-801, Na+, K+-ATPase activity (K+-stimulated p-nitrophenylphosphatase) and high-affinity [H-3]-ouabain binding to membranes from discrete brain regions. Compared to the HTR subgroup, the LTR subgroup presented a lower binding of [H-3]-MK-801 in the hippocampus, frontal cortex and striatum. the subgroups did not differ in K+-p-nitrophenylphosphatase activity, but the LTR subgroup had a lower density of isozymes with a high-affinity to ouabain in the brainstem and in the frontal cortex and a lower affinity to ouabain in the hippocampus than the HTR subgroup. These results suggest that NMDA receptors and ouabain-sensitive Na+, K+-ATPase isozymes may underlie the susceptibility to DMCM-induced convulsions.
Keywords [3H]-ouabain
[3H]-MK 801
Na plus , K plus -ATPase
Convulsion
DMCM
Language English
Sponsor Associacao Fundo de Incentivo a Pesquisa (AFIP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-07-01
Published in Neurochemical Research. New York: Springer/plenum Publishers, v. 37, n. 7, p. 1442-1449, 2012.
ISSN 0364-3190 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1442-1449
Origin http://dx.doi.org/10.1007/s11064-012-0730-4
Access rights Closed access
Type Article
Web of Science ID WOS:000304878200008
URI http://repositorio.unifesp.br/handle/11600/35047

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