Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of alpha-Galactosyl Residues

Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of alpha-Galactosyl Residues

Author Soares, Rodrigo Pedro Google Scholar
Torrecilhas, Ana Claudia Trocoli Autor UNIFESP Google Scholar
Assis, Rafael Ramiro de Google Scholar
Rocha, Marcele Neves Google Scholar
Castro, Felipe Augusto Moura e Google Scholar
Freitas, Gustavo Ferreira de Google Scholar
Murta, Silvane Maria Google Scholar
Santos, Sara Lopes dos Google Scholar
Marques, Alexandre Ferreira Google Scholar
Almeida, Igor Correia de Google Scholar
Romanha, Alvaro Jose Google Scholar
Institution Fiocruz MS
Universidade Federal de São Paulo (UNIFESP)
Univ Texas El Paso
Abstract The glycosylphosphatidylinositol (GPI)-anchored mucins of Trypanosoma cruzi trypomastigotes play an important immunomodulatory role during the course of Chagas disease. Here, some biological activities of tGPI-mucins from four T cruzi isolates, including benznidazole-susceptible (BZS-Y), benznidazole-resistant (BZR-Y), CL, and Colombiana, were evaluated. GPI-mucins were able to differentially trigger the production of interleukin-12 and nitric oxide in BALB/c macrophages and modulate LLC-MK2 cell invasion. the significance of these variations was assessed after analysis of the terminal alpha-galactosyl residues. Enzymatic treatment with alpha-galactosidase indicated a differential expression of O-linked alpha-galactosyl residues among the strains, with higher expression of this sugar in BZS-Y and BZR-Y T cruzi populations followed by Colombiana and CL. Unweighted pair group method analysis of the carbohydrate anchor profile and biological parameters allowed the clustering of two groups. One group includes Y and CL strains (T cruzi II and VI), and the other group is represented by Colombiana strain (T. cruzi I).
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
National Institutes of Health
Biomolecule Analysis Core Facility at the Border Biomedical Research Center/Biology/UTEP University of Texas at El Paso (National Institutes of Health)
Grant number CNPq: 305042/2010-6
CNPq: 552072/2009-5
CNPq: PAPES-IV-400138/2006-9
CNPq: PDJ-150880/2005-7
National Institutes of Health: 1R01AI070655-04
National Institutes of Health: 3R01AI070655-04S1
National Institutes of Health: 2G12RR008124-16A1
National Institutes of Health: 2G12RR008124-16A1S1
Biomolecule Analysis Core Facility at the Border Biomedical Research Center/Biology/UTEP University of Texas at El Paso (National Institutes of Health): 2G12RR008124-16A1
Biomolecule Analysis Core Facility at the Border Biomedical Research Center/Biology/UTEP University of Texas at El Paso (National Institutes of Health): 2G12RR008124-16A1S1
Biomolecule Analysis Core Facility at the Border Biomedical Research Center/Biology/UTEP University of Texas at El Paso (National Institutes of Health): G12MD007592
Date 2012-07-01
Published in American Journal of Tropical Medicine and Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 87, n. 1, p. 87-96, 2012.
ISSN 0002-9637 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Trop Med & Hygiene
Extent 87-96
Origin http://dx.doi.org/10.4269/ajtmh.2012.12-0015
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000306153500016
URI http://repositorio.unifesp.br/handle/11600/35027

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