Food restriction and refeeding induces changes in lipid pathways and fat deposition in the adipose and hepatic tissues in rats with diet-induced obesity

Food restriction and refeeding induces changes in lipid pathways and fat deposition in the adipose and hepatic tissues in rats with diet-induced obesity

Author Duarte, Fernanda Oliveira Google Scholar
Sene-Fiorese, Marcela Google Scholar
Cheik, Nadia Carla Google Scholar
Maria, Adriana Simone Lopes Santa Google Scholar
Aquino, Antonio Eduardo de Google Scholar
Oishi, Jorge Camargo Google Scholar
Rossi, Elizeu Antonio Google Scholar
Duarte, Ana Claudia Garcia de Oliveira Google Scholar
Dâmaso, Ana Raimunda Autor UNIFESP Google Scholar
Institution Universidade Federal de São Carlos (UFSCar)
Universidade Federal de Uberlândia (UFU)
Univ Estadual Paulista
Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of this study was to determine the effects of successive cycles of a moderately restrictive diet and refeeding with a high-fat diet on the metabolism of the adipose and hepatic tissues of obese rats. Rats were assigned to the following groups: a chow diet; a high-fat diet; a moderate caloric restriction; or a moderate caloric restriction plus refeeding. Some animals in each group were given [1-14C]triolein intragastrically, while others received an intraperitoneal injection of 3 mCi 3H2O. All animals were killed by decapitation. the retroperitoneal, visceral epididymal and omental white adipose tissues, brown adipose tissue, liver and blood were immediately removed. the lipid uptake from the diet, in vivo rate of lipogenesis, percentage of fat, lipid profile and leptin concentration were analysed. the high-fat diet promoted an increase in fatty liver (P= 0.05), adiposity mass (P= 0.05) and the plasma concentration of leptin (P= 0.05) and a decreased lipid uptake in white adipose tissue depots (P= 0.05) in relation to the chow diet. the moderate caloric restriction did not reverse the changes promoted by the high-fat diet but induced a small decrease in adiposity, which was reversed after refeeding, and the animals maintained a dyslipidaemic profile and high fat deposition in the liver. We can conclude that the high-fat diet and subsequent moderate caloric restriction plus refeeding increased the risks of developing visceral obesity, dyslipidaemia and non-alcoholic fatty liver disease, which suggests that this type of experimental protocol can be used to study mechanisms related to the metabolic syndrome.
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number CNPq: 141486/2003-3
FAPESP: 99/12981-7
Date 2012-07-01
Published in Experimental Physiology. Hoboken: Wiley-Blackwell, v. 97, n. 7, p. 882-894, 2012.
ISSN 0958-0670 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 882-894
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000306281700007

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