Distinct localization of T cell Agrin during antigen presentation - evidence for the expression of Agrin receptor(s) in antigen-presenting cells

Distinct localization of T cell Agrin during antigen presentation - evidence for the expression of Agrin receptor(s) in antigen-presenting cells

Autor Kabouridis, Panagiotis S. Google Scholar
Pimentel, Tatiana A. Autor UNIFESP Google Scholar
Brancaleone, Vincenzo Google Scholar
D'Acquisto, Fulvio Google Scholar
Oliani, Sonia M. Autor UNIFESP Google Scholar
Perretti, Mauro Google Scholar
Jury, Elizabeth C. Google Scholar
Instituição Queen Mary Univ London
Universidade Federal de São Paulo (UNIFESP)
Univ Basilicata
São Paulo State Univ UNESP
UCL
Resumo Agrin is over-expressed by activated and autoimmune T cells, and synergizes with the T cell receptor (TCR) to augment cell activation. in the present study, we show that Agrin accumulates to distinct areas of the plasma membrane and that cell activation causes its redistribution. During antigen presentation, Agrin primarily accumulates to the periphery of the mature immunological synapse, mostly in lamellipodia-like protrusions that wrap around the antigen-presenting cell and, conversely, anti-Agrin sera induced a significant redistribution of TCR at the plasma membrane. We also provide evidence for the expression of Agrin receptors in peripheral blood monocytes, dendritic cells and a fraction of B cells. Interestingly, interferon-a treatment, which induces the expression of Agrin in T cells, also augmented Agrin binding to monocytes. Stimulation of monocytes with recombinant Agrin induced the clustering of surface receptors, including major histocompatibility complex class II, activation of intracellular signalling cascades, as well as enhanced dsRNA-induced expression of pro-inflammatory cytokines interleukin-6 and tumour necrosis factor-a. Collectively, these results confirm the location of Agrin at the immunological synapse between T cells and antigen-presenting cells and justify further characterization of its receptors in the immune system.
Assunto Agrin
Agrin receptor
antigen-presenting cells
immunological synapse
T lymphocytes
Idioma Inglês
Financiador Arthritis Research UK
Número do financiamento Arthritis Research UK: 18106
Arthritis Research UK: 16018
Data 2012-07-01
Publicado em Febs Journal. Hoboken: Wiley-Blackwell, v. 279, n. 13, p. 2368-2380, 2012.
ISSN 1742-464X (Sherpa/Romeo, fator de impacto)
Editor Wiley-Blackwell
Extensão 2368-2380
Fonte http://dx.doi.org/10.1111/j.1742-4658.2012.08615.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000305279500010
URI http://repositorio.unifesp.br/handle/11600/35018

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