Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle

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dc.contributor.author Lima, Cilene Reboucas de [UNIFESP]
dc.contributor.author Santos Júnior, José de Arimatea [UNIFESP]
dc.contributor.author Pinto Nazário, Afonso Celso [UNIFESP]
dc.contributor.author Michelacci, Yara Maria [UNIFESP]
dc.date.accessioned 2016-01-24T14:27:23Z
dc.date.available 2016-01-24T14:27:23Z
dc.date.issued 2012-07-01
dc.identifier http://dx.doi.org/10.1016/j.bbagen.2012.04.010
dc.identifier.citation Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1820, n. 7, p. 1009-1019, 2012.
dc.identifier.issn 0304-4165
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35010
dc.description.abstract Background: Fibroadenoma is the most common breast tumor in young women, and its growth and metabolism may be under hormonal control. in the present paper we described the proteoglycan (PG) composition and synthesis rate of normal breast and fibroadenoma during the menstrual cycle.Methods: Samples of fibroadenoma and adjacent normal breast tissue were obtained at surgery. PGs were characterized by agarose gel electrophoresis and enzymatic degradation with glycosaminoglycan (GAG) lyases, and immunolocalized by confocal microscopy. To assess the synthesis rate, PGs were metabolic labeled by S-35-sulfate.Results: the concentration of PGs in normal breast was higher during the secretory phase. Fibroadenoma contained and synthesized more PGs than their paired controls, but the PG concentrations varied less with the menstrual cycle and, in contrast to normal tissue, peaked in the proliferative phase. the main mammary GAGs are heparan sulfate (HS, 71%-74%) and dermatan sulfate (DS, 26%-29%). the concentrations of both increased in fibroadenoma, but DS increased more, becoming 35%-37% of total. the DS chains contained more beta-D-glucuronic acid (IdoUA/GlcUA ratios were >10 in normal breast and 2-7 in fibroadenoma). the S-35-sulfate incorporation rate revealed that the in vitro synthesis rate of DS was higher than HS. Decorin was present in both tissues, while versican was found only in fibroadenoma.Conclusions: in normal breast, the PG concentration varied with the menstrual cycle. It was increased in fibroadenoma, especially DS.General significancePGs are increased in fibroadenoma, but their concentrations may be less sensitive to hormonal control. (C) 2012 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Sociedade Paulista para o Desenvolvimento da Medicina (SPDM), São Paulo, SP, Brazil
dc.format.extent 1009-1019
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Biochimica Et Biophysica Acta-general Subjects
dc.rights Acesso aberto
dc.subject Fibroadenoma en
dc.subject Proteoglycan en
dc.subject Menstrual cycle en
dc.subject Normal breast en
dc.subject Decorin en
dc.subject Versican en
dc.title Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo UNIFESP, Dept Bioquim, Escola Paulista Med, Disciplina Biol Mol, BR-04044020 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo UNIFESP, Dept Ginecol, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP, Dept Bioquim, Escola Paulista Med, Disciplina Biol Mol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP, Dept Ginecol, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.identifier.file WOS000305366100026.pdf
dc.identifier.doi 10.1016/j.bbagen.2012.04.010
dc.description.source Web of Science
dc.identifier.wos WOS:000305366100026



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