Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle

Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle

Autor Lima, Cilene Reboucas de Autor UNIFESP Google Scholar
Santos Júnior, José de Arimatea Autor UNIFESP Google Scholar
Pinto Nazário, Afonso Celso Autor UNIFESP Google Scholar
Michelacci, Yara Maria Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Background: Fibroadenoma is the most common breast tumor in young women, and its growth and metabolism may be under hormonal control. in the present paper we described the proteoglycan (PG) composition and synthesis rate of normal breast and fibroadenoma during the menstrual cycle.Methods: Samples of fibroadenoma and adjacent normal breast tissue were obtained at surgery. PGs were characterized by agarose gel electrophoresis and enzymatic degradation with glycosaminoglycan (GAG) lyases, and immunolocalized by confocal microscopy. To assess the synthesis rate, PGs were metabolic labeled by S-35-sulfate.Results: the concentration of PGs in normal breast was higher during the secretory phase. Fibroadenoma contained and synthesized more PGs than their paired controls, but the PG concentrations varied less with the menstrual cycle and, in contrast to normal tissue, peaked in the proliferative phase. the main mammary GAGs are heparan sulfate (HS, 71%-74%) and dermatan sulfate (DS, 26%-29%). the concentrations of both increased in fibroadenoma, but DS increased more, becoming 35%-37% of total. the DS chains contained more beta-D-glucuronic acid (IdoUA/GlcUA ratios were >10 in normal breast and 2-7 in fibroadenoma). the S-35-sulfate incorporation rate revealed that the in vitro synthesis rate of DS was higher than HS. Decorin was present in both tissues, while versican was found only in fibroadenoma.Conclusions: in normal breast, the PG concentration varied with the menstrual cycle. It was increased in fibroadenoma, especially DS.General significancePGs are increased in fibroadenoma, but their concentrations may be less sensitive to hormonal control. (C) 2012 Elsevier B.V. All rights reserved.
Assunto Fibroadenoma
Proteoglycan
Menstrual cycle
Normal breast
Decorin
Versican
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Sociedade Paulista para o Desenvolvimento da Medicina (SPDM), São Paulo, SP, Brazil
Data 2012-07-01
Publicado em Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1820, n. 7, p. 1009-1019, 2012.
ISSN 0304-4165 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 1009-1019
Fonte http://dx.doi.org/10.1016/j.bbagen.2012.04.010
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000305366100026
URI http://repositorio.unifesp.br/handle/11600/35010

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