Hypothalamic expression of Peg3 gene is associated with maternal care differences between SM/J and LG/J mouse strains

Hypothalamic expression of Peg3 gene is associated with maternal care differences between SM/J and LG/J mouse strains

Author Chiavegatto, Silvana Google Scholar
Sauce, Bruno Google Scholar
Ambar, Guilherme Google Scholar
Cheverud, James M. Google Scholar
Peripato, Andrea Cristina Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Natl Inst Dev Psychiat Childhood & Adolescence IN
Universidade Federal de São Carlos (UFSCar)
Washington Univ
Universidade Federal de São Paulo (UNIFESP)
Abstract Maternal care is essential in mammals, and variations in the environment provided by mothers may directly influence the viability of newborns and emotional behavior later in life. A previous study investigated genetic variations associated with maternal care in an intercross of LG/J and SM/J inbred mouse strains and identified two single-locus QTLs (quantitative trait loci). Here, we selected three candidate genes located within these QTLs intervals; Oxt on chromosome 2, and FosB and Peg3 on chromosome 7 and tested their association with maternal care. LG/J females showed impaired postpartum nest building and pup retrieval, a one-day delay in milk ejection, reduced exploratory activity, and higher anxiety-like behavior when compared to SM/J females. the nucleotide sequences of Oxt and FosB were similar between strains, as were their hypothalamic expression levels. Conversely, Peg3 nucleotide sequences showed four nonsynonymous replacement substitutions on LG/J dams, T11062G, G13744A, A13808G, and G13813A, and a 30 base pair (10 aa) in tandem repeat in the coding region with three copies in SM/J and five copies in LG/J. Maternal care impaired LG/J mothers express 37% lower Peg3 mRNA levels in the hypothalamus on the second postpartum day. We also found an association of the Peg3 repeat-variant and poor maternal care in F-2 heterozygote females derived from a LG/J x SM/J intercross. These results may suggest that the maternally imprinted Peg3 gene is responsible for the single-locus QTL on chromosome 7 that has been shown to influence maternal care in these strains. Furthermore, these data provide additional support for an epigenetic regulation of maternal behavior.
Keywords Chromosome
epigenetic
FosB
gene expression
gene variation
hypothalamus
imprinting
maternal behavior
Oxt
QTL
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 09/01333-8
FAPESP: 04/14583-9
FAPESP: 05/56353-2
Date 2012-07-01
Published in Brain and Behavior. Hoboken: John Wiley & Sons Inc, v. 2, n. 4, p. 365-376, 2012.
ISSN 2162-3279 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 365-376
Origin http://dx.doi.org/10.1002/brb3.58
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000209174100002
URI http://repositorio.unifesp.br/handle/11600/35007

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