Influence of estrogen replacement and aging on the expression of nerve growth factor in the urethra of female rats

Influence of estrogen replacement and aging on the expression of nerve growth factor in the urethra of female rats

Author Zucchi, Eliana V. M. Autor UNIFESP Google Scholar
Jarmy-Di Bella, Zsuzsanna I. K. Autor UNIFESP Google Scholar
Castro, Rodrigo A. Autor UNIFESP Google Scholar
Takano, Claudia C. Autor UNIFESP Google Scholar
Simoes, Manuel J. Autor UNIFESP Google Scholar
Girao, Manoel J. B. C. Autor UNIFESP Google Scholar
Sartori, Marair G. F. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Objective To evaluate the expression of nerve growth factor (NGF) in the urethra of adult female rats in different hormonal status using immunohistochemical assay. Methods Forty-eight rats (Rattus norvegicus albinus, Rodentia, Mammalia) from the CEDEME-UNIFESP laboratory animal facility were used in the study. Rats were divided into four groups: group A, 12 non-neutered rats; group B, 12 oophorectomized rats; group C, 12 castrated rats treated with 17 beta-estradiol for 30 days; and group D, 12 aging rats. Animals were killed by lethal injection and their urethra was removed. NGF expression was evaluated by means of immunohistochemistry using mouse monoclonal primary IgG antibody anti-NGF diluted 1:600, and read under 400 x magnification. Digital analysis of the images was done by Imagelab software. the intensity of the dark brown color was used as a measure of NGF cytoplasmatic expression, and was used to quantify the percentage of epithelial and muscular layer cells showing this neurotrophin. Results After oophorectomy, rats showed a significant increase in NGF expression in the periurethral muscular layer. Compared with oophorectomized rats, NGF expression increased in the epithelial layer and diminished in the periurethral smooth muscle following estrogen administration. in 18-month-old rats, NGF expression was diminished in both epithelial and muscular layers. Conclusions Hormonal status led to significant differences in NGF protein expression in urethral epithelium and periurethral smooth muscle. Neurourol. Urodynam. 31:702705, 2012. (C) 2012 Wiley Periodicals, Inc.
Keywords estrogen
low urinary tract
nerve growth factor (NGF)
Language English
Date 2012-06-01
Published in Neurourology and Urodynamics. Hoboken: Wiley-Blackwell, v. 31, n. 5, p. 702-705, 2012.
ISSN 0733-2467 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 702-705
Origin http://dx.doi.org/10.1002/nau.21174
Access rights Closed access
Type Article
Web of Science ID WOS:000305389800019
URI http://repositorio.unifesp.br/handle/11600/34934

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