Consequences of pilocarpine-induced status epilepticus in immunodeficient mice

Consequences of pilocarpine-induced status epilepticus in immunodeficient mice

Author Vignoli, Thiago Autor UNIFESP Google Scholar
Nehlig, Astrid Google Scholar
Massironi, Silvia Gomes Google Scholar
Sinigaglia Coimbra, Rita de Cassia Autor UNIFESP Google Scholar
Naffah Mazzacoratti, Maria da Graca Autor UNIFESP Google Scholar
Silva, Iara Ribeiro Autor UNIFESP Google Scholar
Castro Neto, Eduardo Ferreira de Autor UNIFESP Google Scholar
Persike, Daniele Suzete Autor UNIFESP Google Scholar
Silva Fernandes, Maria Jose da Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Strasbourg
Universidade de São Paulo (USP)
Abstract Systemic injection of pilocarpine in rodents induces status epilepticus (SE) and reproduces the main characteristics of temporal lobe epilepsy (TLE). Different mechanisms are activated by SE contributing to cell death and immune system activation. We used BALB/c nude mice, a mutant that is severely immunocompromised, to characterize seizure pattern, neurochemical changes, cell death and c-Fos activation secondarily to pilocarpine-induced SE. the behavioral seizures were less severe in BALB/c nude than in BALB/c wild type mice. However, nude mice presented more tonic clonic episodes and higher mortality rate during SE. the c-Fos expression was most prominent in the caudate-putamen, CA3 (p < 0.05), dentate gyrus, entorhinal cortex (p < 0.001), basolateral nucleus of amygdala (p < 0.01) and piriform cortex (p < 0.05) of BALB/c nude mice than of BALB/c. Besides, nude mice subjected to SE presented high number of Fluorojade-B (FJB) stained cells in the piriform cortex, amygdala (p < 0.05) and hilus (p < 0.05) in comparison with BALB/c mice. A significant increase in the level of glutamate and GABA was found in the hippocampus and cortex of BALB/c mice presenting SE in comparison to controls. However, the level of glutamate was higher in the brains of BALB nude mice than in the brains of BALB/c wild type mice, while the levels of GABA were unchanged. These results indicate that the brains of immunodeficient nude mice are more vulnerable to the deleterious effects of pilocarpine-induced SE as they present intense activation, increased glutamate levels and more cell death. Published by Elsevier B.V.
Keywords BALB/c nude mice
Status epilepticus
Pilocarpine
c-Fos, amino acid
Cell death
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
INNT/MCT
Date 2012-04-23
Published in Brain Research. Amsterdam: Elsevier B.V., v. 1450, p. 125-137, 2012.
ISSN 0006-8993 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 125-137
Origin http://dx.doi.org/10.1016/j.brainres.2012.02.028
Access rights Closed access
Type Article
Web of Science ID WOS:000303224600013
URI http://repositorio.unifesp.br/handle/11600/34803

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