Grape juice concentrate modulates p16 expression in high fat diet-induced liver steatosis in Wistar rats

Grape juice concentrate modulates p16 expression in high fat diet-induced liver steatosis in Wistar rats

Author Ferreira, Andressa Orlandeli Autor UNIFESP Google Scholar
Boiago Golluecke, Andrea Pittelli Google Scholar
Noguti, Juliana Autor UNIFESP Google Scholar
Pereira da Silva, Victor Hugo Autor UNIFESP Google Scholar
Hojo Yamamura, Elsa Tiemi Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Catolica Santos
Abstract Purpose: the goal of this study was to investigate whether subchronic treatment with grape juice concentrate is able to protect the liver from high fat diet injury in rats. the effects of grape juice concentrate treatment on histopathological changes, and immunohistochemistry for p53, p16 and p21 were evaluated.Methods: Male Wistar rats (n = 18) were distributed into three groups: group 1: negative control; group 2: cholesterol at 1% (w/w) in their diet, treated during 5 weeks; and group 3: cholesterol at 1% in their chow during 5 weeks, and grape juice concentrate at 222 mg per day in their drinking-water in the last week only.Results: the results pointed out that treatment with grape juice concentrate did not show remarkable differences regarding liver tissue in the cholesterol-exposed group when compared to group 2. However, grape juice concentrate was able to modulate p16 immunoexpression when compared to high fat diet group. p53 and p21 did not show any significant statistical differences among groups.Conclusion: Taken together, our results suggest that subchronic grape juice concentrate administration was able to modulate cell cycle control by downregulation of p16 immunoexpression in high fat diet-induced liver steatosis in rats.
Keywords Cholesterol diet
rats
grape juice
p53
p16
p21
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-04-01
Published in Toxicology Mechanisms and Methods. London: Informa Healthcare, v. 22, n. 3, p. 218-224, 2012.
ISSN 1537-6516 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 218-224
Origin http://dx.doi.org/10.3109/15376516.2011.631196
Access rights Closed access
Type Article
Web of Science ID WOS:000300254700007
URI http://repositorio.unifesp.br/handle/11600/34790

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