Inactivation and fragmentation of lectin from Bothrops leucurus snake venom by gamma irradiation

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dc.contributor.author Nunes, E. S.
dc.contributor.author Souza, M. A. A.
dc.contributor.author Vaz, A. F. M.
dc.contributor.author Coelho, L. C. B. B.
dc.contributor.author Aguiar, J. S.
dc.contributor.author Silva, T. G.
dc.contributor.author Guarnieri, M. C.
dc.contributor.author Melo, A. M. M. A.
dc.contributor.author Oliva, M. L. V. [UNIFESP]
dc.contributor.author Correia, M. T. S.
dc.date.accessioned 2016-01-24T14:27:04Z
dc.date.available 2016-01-24T14:27:04Z
dc.date.issued 2012-04-01
dc.identifier http://dx.doi.org/10.1016/j.radphyschem.2011.12.048
dc.identifier.citation Radiation Physics and Chemistry. Oxford: Pergamon-Elsevier B.V., v. 81, n. 4, p. 484-487, 2012.
dc.identifier.issn 0969-806X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/34764
dc.description.abstract Gamma radiation alters the molecular structure of biomolecules and is able to mitigate the action of snake venoms and their isolated toxins. the effect of gamma-radiation on the folding of Bothrops lecurus venom lectin was measured by a hemagglutinating assay, intrinsic and bis-ANS fluorescence. Intrinsic and bis-ANS fluorescence analyses indicated that irradiation caused unfolding followed by aggregation of the lectin. Our results suggest that irradiation can lead to significant changes in the protein structure, which may promote the loss of its binding property and toxic action. (C) 2012 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 484-487
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Radiation Physics and Chemistry
dc.rights Acesso restrito
dc.subject Bothrops leucurus en
dc.subject Lectin en
dc.subject Gamma rays en
dc.subject Snake venom en
dc.title Inactivation and fragmentation of lectin from Bothrops leucurus snake venom by gamma irradiation en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de Pernambuco (UFPE)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Pernambuco, Dept Bioquim, BR-50670420 Recife, PE, Brazil
dc.description.affiliation Univ Fed Pernambuco, Dept Antibiot, BR-50670420 Recife, PE, Brazil
dc.description.affiliation Univ Fed Pernambuco, Dept Zool, BR-50670420 Recife, PE, Brazil
dc.description.affiliation Univ Fed Pernambuco, Dept Biofis & Radiobiol, BR-50670420 Recife, PE, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/j.radphyschem.2011.12.048
dc.description.source Web of Science
dc.identifier.wos WOS:000301016100022



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