Inactivation and fragmentation of lectin from Bothrops leucurus snake venom by gamma irradiation

Inactivation and fragmentation of lectin from Bothrops leucurus snake venom by gamma irradiation

Author Nunes, E. S. Google Scholar
Souza, M. A. A. Google Scholar
Vaz, A. F. M. Google Scholar
Coelho, L. C. B. B. Google Scholar
Aguiar, J. S. Google Scholar
Silva, T. G. Google Scholar
Guarnieri, M. C. Google Scholar
Melo, A. M. M. A. Google Scholar
Oliva, M. L. V. Autor UNIFESP Google Scholar
Correia, M. T. S. Google Scholar
Institution Universidade Federal de Pernambuco (UFPE)
Universidade Federal de São Paulo (UNIFESP)
Abstract Gamma radiation alters the molecular structure of biomolecules and is able to mitigate the action of snake venoms and their isolated toxins. the effect of gamma-radiation on the folding of Bothrops lecurus venom lectin was measured by a hemagglutinating assay, intrinsic and bis-ANS fluorescence. Intrinsic and bis-ANS fluorescence analyses indicated that irradiation caused unfolding followed by aggregation of the lectin. Our results suggest that irradiation can lead to significant changes in the protein structure, which may promote the loss of its binding property and toxic action. (C) 2012 Elsevier B.V. All rights reserved.
Keywords Bothrops leucurus
Lectin
Gamma rays
Snake venom
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-04-01
Published in Radiation Physics and Chemistry. Oxford: Pergamon-Elsevier B.V., v. 81, n. 4, p. 484-487, 2012.
ISSN 0969-806X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 484-487
Origin http://dx.doi.org/10.1016/j.radphyschem.2011.12.048
Access rights Closed access
Type Article
Web of Science ID WOS:000301016100022
URI http://repositorio.unifesp.br/handle/11600/34764

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