Alkylation-induced genotoxicity as a predictor of DNA repair deficiency following experimental oral carcinogenesis

Alkylation-induced genotoxicity as a predictor of DNA repair deficiency following experimental oral carcinogenesis

Author Carvalho, Juliana G. Autor UNIFESP Google Scholar
Noguti, Juliana Autor UNIFESP Google Scholar
Silva, Victor Hugo P. da Autor UNIFESP Google Scholar
Dedivitis, Rogerio A. Google Scholar
Franco, Marcello Autor UNIFESP Google Scholar
Ribeiro, Daniel A. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Ana Costa Hosp & Santa Casa Santos
Abstract The aim of this study was to evaluate alkylation induced genotoxicity as a result of DNA repair deficiency during 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis by means of single cell gel (comet) assay. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks. Ten animals were used as negative control. Blood samples and oral mucosa cells collected from all animals were divided into two aliquots of 20 mu L each to study basal DNA damage and DNA damage due to genotoxin sensitivity. the first aliquot was processed immediately for comet assay to assess basal DNA damage. the second aliquot was treated with a known genotoxin, methylmetanesulfonate. Significantly greater DNA damage was noticed to oral mucosa cells from 4, and 12 weeks post-treatment. Peripheral blood cells did show statistically significant differences (P < 0.05) after 20 weeks-group (squamous cell carcinoma). in conclusion, alkylation induced genotoxicity as a result of DNA repair deficiency is present in oral mucosa cells following oral experimental carcinogenesis.
Keywords Rat tongue mucosa
Oral squamous cell carcinoma
4-Nitroquinoline 1-oxide
DNA repair deficiency
Methylmetanesulfonate
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-04-01
Published in Journal of Molecular Histology. Dordrecht: Springer, v. 43, n. 2, p. 145-150, 2012.
ISSN 1567-2379 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 145-150
Origin http://dx.doi.org/10.1007/s10735-011-9388-5
Access rights Closed access
Type Article
Web of Science ID WOS:000303357100003
URI http://repositorio.unifesp.br/handle/11600/34750

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