Acute cocaine treatment increases thimet oligopeptidase in the striatum of rat brain

Acute cocaine treatment increases thimet oligopeptidase in the striatum of rat brain

Author Dalio, Fernanda Montiel Autor UNIFESP Google Scholar
Visniauskas, Bruna Autor UNIFESP Google Scholar
Bicocchi, Eliane S. Google Scholar
Perry, Juliana Cini Autor UNIFESP Google Scholar
Freua, Rodrigo Autor UNIFESP Google Scholar
Gesteira, Tarsis Ferreira Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Machado, Mauricio Ferreira Marcondes Autor UNIFESP Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Ferro, Emer Suavinho Autor UNIFESP Google Scholar
Andersen, Monica Levy Autor UNIFESP Google Scholar
Toledo, Claudio A. B. Google Scholar
Chagas, Jair Ribeiro Autor UNIFESP Google Scholar
Oliveira, Vitor Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Cidade São Paulo UNICID
Universidade de São Paulo (USP)
Abstract Many studies indicate that thimet oligopeptidase (EC3.4.24.15; TOP) can be implicated in the metabolism of bioactive peptides, including dynorphin 1-8, alpha-neoendorphin, beta-neoendorphin and GnRH. Furthermore, the higher levels of this peptidase are found in neuroendocrine tissue and testis. in the present study, we have evaluated the effect of acute cocaine administration in male rats on TOP specific activity and mRNA levels in prosencephalic brain areas related with the reward circuitry; ventral striatum, hippocampus, and frontal cortex. No significant differences on TOP specific activity were detected in the hippocampus and frontal cortex of cocaine treated animals compared to control vehicle group. However, a significant increase in activity was observed in the ventral striatum of cocaine treated-rats. the increase occurred in both, TOP specific activity and TOP relative mRNA amount determined by real time RT-PCR. As TOP can be implicated in the processing of many neuropeptides, and previous studies have shown that cocaine also alters the gene expression of proenkephalin and prodynorphin in the striatum, the present findings suggest that TOP changes in the brain could play important role in the balance of neuropeptide level correlated with cocaine effects. (C) 2012 Elsevier Inc. All rights reserved.
Keywords Cocaine
Thimet oligopeptidase
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Associacao de Fundo e Incentivo a Pesquisa (AFIP)
Grant number FAPESP: 98/14303-3
CNPq: 553645/2009-9
CNPq: 558924/2008-5
CNPq: 481104/2004-6
CNPq: 306587/2010-6
Date 2012-03-23
Published in Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 419, n. 4, p. 724-727, 2012.
ISSN 0006-291X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 724-727
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000302335800024

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