Annexin A1 protein attenuates cyclosporine-induced renal hemodynamics changes and macrophage infiltration in rats

Annexin A1 protein attenuates cyclosporine-induced renal hemodynamics changes and macrophage infiltration in rats

Author Araujo, Leandro Pires Autor UNIFESP Google Scholar
Truzzi, Renata Ramos Google Scholar
Florido Mendes, Gloria Elisa Google Scholar
Mendes Luz, Marcus Alexandre Google Scholar
Burdmann, Emmanuel A. Google Scholar
Oliani, Sonia Maria Autor UNIFESP Google Scholar
Institution São Paulo State Univ UNESP
Universidade Federal de São Paulo (UNIFESP)
Sao Jose do Rio Preto Med Sch
Universidade de São Paulo (USP)
Abstract Cyclosporine (CsA) remains an important immunosuppressant for transplantation and for treatment of autoimmune diseases. the most troublesome side effect of CsA is renal injury. Acute CsA-induced nephrotoxicity is characterized by reduced renal blood flow (RBF) and glomerular filtration rate (GFR) due to afferent arteriole vasoconstriction. Annexin A1 (ANXA1) is a potent anti-inflammatory protein with protective effect in renal ischemia/reperfusion injury. Here we study the effects of ANXA1 treatment in an experimental model of acute CsA nephrotoxicity.Salt-depleted rats were randomized to treatment with VH (vehicles 1 mL/kg body weight/day), ANXA1 (Ac2-26 peptide 1 mg/kg body weight/day intraperitoneally), CsA (20 mg/kg body weight/day subcutaneously) and CsA + ANXA1 (combination) for seven days. We compared renal function and hemodynamics, renal histopathology, renal tissue macrophage infiltration and renal ANXA1 expression between the four groups.CsA significantly impaired GFR and RBF, caused tubular dilation and macrophage infiltration and increased ANXA1 renal tissue expression. Treatment with ANXA1 attenuated CSA-induced hemodynamic changes, tubular injury and macrophage infiltration.ANXA1 treatment attenuated renal hemodynamic injury and inflammation in an acute CsA nephrotoxicity model.
Keywords Acute renal injury
Annexin A1
Cyclosporine nephrotoxicity
Immunosuppression
Inflammation
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2008/01,048-9
CNPq: 306,074/2007-9
CNPq: 307,371/2006-9
Date 2012-03-01
Published in Inflammation Research. Basel: Springer Basel Ag, v. 61, n. 3, p. 189-196, 2012.
ISSN 1023-3830 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 189-196
Origin http://dx.doi.org/10.1007/s00011-011-0400-z
Access rights Closed access
Type Article
Web of Science ID WOS:000300292100002
URI http://repositorio.unifesp.br/handle/11600/34657

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