Strength Training with Blood Flow Restriction Diminishes Myostatin Gene Expression

Strength Training with Blood Flow Restriction Diminishes Myostatin Gene Expression

Author Laurentino, Gilberto Candido Google Scholar
Ugrinowitsch, Carlos Google Scholar
Roschel, Hamilton Google Scholar
Aoki, Marcelo Saldanha Google Scholar
Soares, Antonio Garcia Google Scholar
Neves, Manoel Google Scholar
Aihara, Andre Yui Autor UNIFESP Google Scholar
Fernandes, Artur da Rocha Correa Autor UNIFESP Google Scholar
Tricoli, Valmor Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract LAURENTINO, G. C., C. UGRINOWITSCH, H. ROSCHEL, M. S. AOKI, A. G. SOARES, M. NEVES JR, A. Y. AIHARA, A. DA ROCHA CORREA FERNANDES, and V. TRICOLI. Strength Training with Blood Flow Restriction Diminishes Myostatin Gene Expression. Med. Sci. Sports Exerc., Vol. 44, No. 3, pp. 406-412, 2012. Purpose: the aim of the study was to determine whether the similar muscle strength and hypertrophy responses observed after either low-intensity resistance exercise associated with moderate blood flow restriction or high-intensity resistance exercise are associated with similar changes in messenger RNA (mRNA) expression of selected genes involved in myostatin (MSTN) signaling. Methods: Twenty-nine physically active male subjects were divided into three groups: low-intensity (20% one-repetition maximum (1RM)) resistance training (LI) (n = 10), low-intensity resistance exercise associated with moderate blood flow restriction (LIR) (n = 10), and high-intensity (80% 1RM) resistance exercise (HI) (n = 9). All of the groups underwent an 8-wk training program. Maximal dynamic knee extension strength (1RM), quadriceps cross-sectional area (CSA), MSTN, follistatin-like related genes (follistatin (FLST), follistatin-like 3 (FLST-3)), activin IIb, growth and differentiation factor-associated serum protein 1 (GASP-1), and MAD-related protein (SMAD-7) mRNA gene expression were assessed before and after training. Results: Knee extension 1RM significantly increased in all groups (LI = 20.7%, LIR = 40.1%, and HI = 36.2%). CSA increased in both the LIR and HI groups (6.3% and 6.1%, respectively). MSTN mRNA expression decreased in the LIR and HI groups (45% and 41%, respectively). There were no significant changes in activin IIb (P > 0.05). FLST and FLST-3 mRNA expression increased in all groups from pre- to posttest (P < 0.001). FLST-3 expression was significantly greater in the HI when compared with the LIR and LI groups at posttest (P = 0.024 and P = 0.018, respectively). GASP-1 and SMAD-7 gene expression significantly increased in both the LIR and HI groups. Conclusions: We concluded that LIR was able to induce gains in 1RM and quadriceps CSA similar to those observed after traditional HI. These responses may be related to the concomitant decrease in MSTN and increase in FLST isoforms, GASP-1, and SMAD-7 mRNA gene expression.
Keywords RESISTANCE TRAINING
SKELETAL MUSCLE HYPERTROPHY
MUSCLE BIOPSY
VASCULAR OCCLUSION
HYPOXIA
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2009/09820-5
FAPESP: 2010/51428-2
CNPq: 470207/2008-6
CNPq: 303162/2008-2
Date 2012-03-01
Published in Medicine and Science in Sports and Exercise. Philadelphia: Lippincott Williams & Wilkins, v. 44, n. 3, p. 406-412, 2012.
ISSN 0195-9131 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 406-412
Origin http://dx.doi.org/10.1249/MSS.0b013e318233b4bc
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000300639500006
URI http://repositorio.unifesp.br/handle/11600/34646

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