Crataeva tapia bark lectin is an affinity adsorbent and insecticidal agent

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dc.contributor.author Sousa de Araujo, Regina Maria
dc.contributor.author Ferreira, Rodrigo da Silva [UNIFESP]
dc.contributor.author Napoleao, Thiago Henrique
dc.contributor.author Carneiro-da-Cunha, Maria das Gracas
dc.contributor.author Breitenbach Barroso Coelho, Luana Cassandra
dc.contributor.author Santos Correia, Maria Tereza dos
dc.contributor.author Oliva, Maria Luiza Vilela [UNIFESP]
dc.contributor.author Guedes Paiva, Patricia Maria
dc.date.accessioned 2016-01-24T14:17:53Z
dc.date.available 2016-01-24T14:17:53Z
dc.date.issued 2012-02-01
dc.identifier http://dx.doi.org/10.1016/j.plantsci.2011.10.018
dc.identifier.citation Plant Science. Clare: Elsevier B.V., v. 183, p. 20-26, 2012.
dc.identifier.issn 0168-9452
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/34593
dc.description.abstract Hemagglutinating activity has been associated to presence of lectin, carbohydrate-binding proteins. in this work Crataeva tapia bark lectin (CrataBL) was purified in milligram quantities (28 mg per g of bark) by ion exchange chromatography. the lectin was thermo-stable, ion-independent and N-terminal sequence analysis demonstrated similarity with miraculin and miraculin-like proteins (plant defensive proteins). Glycosylated nature of CrataBL was revealed using glycoprotein staining (periodic acid-Schiff's reagent), positive for polypeptides of apparent molecular masses 21 and 40 kDa on SOS-PAGE. Gel diffusion assay showed that glucose/mannose isolectins from Cratylia mollis recognized CrataBL glycan moiety. CrataBL hemagglutinating activity was inhibited by glycoproteins and CrataBL immobilized on cyanogen bromide-activated sepharose 4B (1 mL) bound 0.54 mg of glycoprotein (casein, fetuin and ovalbumin) per cycle. CrataBL was an insecticide agent against Nasutitermes corniger workers (termite that attack woods) with LC50 of 0.475 mg mL(-1) for 6 days. (C) 2011 Elsevier Ireland Ltd. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
dc.format.extent 20-26
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Plant Science
dc.rights Acesso restrito
dc.subject Crataeva tapia en
dc.subject Bark lectin en
dc.subject Affinity matrix en
dc.subject Termiticidal activity en
dc.title Crataeva tapia bark lectin is an affinity adsorbent and insecticidal agent en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de Pernambuco (UFPE)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Pernambuco, Dept Bioquim CCB, BR-50670420 Recife, PE, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/j.plantsci.2011.10.018
dc.description.source Web of Science
dc.identifier.wos WOS:000299713300004



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