Crataeva tapia bark lectin is an affinity adsorbent and insecticidal agent

Crataeva tapia bark lectin is an affinity adsorbent and insecticidal agent

Author Sousa de Araujo, Regina Maria Google Scholar
Ferreira, Rodrigo da Silva Autor UNIFESP Google Scholar
Napoleao, Thiago Henrique Google Scholar
Carneiro-da-Cunha, Maria das Gracas Google Scholar
Breitenbach Barroso Coelho, Luana Cassandra Google Scholar
Santos Correia, Maria Tereza dos Google Scholar
Oliva, Maria Luiza Vilela Autor UNIFESP Google Scholar
Guedes Paiva, Patricia Maria Google Scholar
Institution Universidade Federal de Pernambuco (UFPE)
Universidade Federal de São Paulo (UNIFESP)
Abstract Hemagglutinating activity has been associated to presence of lectin, carbohydrate-binding proteins. in this work Crataeva tapia bark lectin (CrataBL) was purified in milligram quantities (28 mg per g of bark) by ion exchange chromatography. the lectin was thermo-stable, ion-independent and N-terminal sequence analysis demonstrated similarity with miraculin and miraculin-like proteins (plant defensive proteins). Glycosylated nature of CrataBL was revealed using glycoprotein staining (periodic acid-Schiff's reagent), positive for polypeptides of apparent molecular masses 21 and 40 kDa on SOS-PAGE. Gel diffusion assay showed that glucose/mannose isolectins from Cratylia mollis recognized CrataBL glycan moiety. CrataBL hemagglutinating activity was inhibited by glycoproteins and CrataBL immobilized on cyanogen bromide-activated sepharose 4B (1 mL) bound 0.54 mg of glycoprotein (casein, fetuin and ovalbumin) per cycle. CrataBL was an insecticide agent against Nasutitermes corniger workers (termite that attack woods) with LC50 of 0.475 mg mL(-1) for 6 days. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
Keywords Crataeva tapia
Bark lectin
Affinity matrix
Termiticidal activity
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
Date 2012-02-01
Published in Plant Science. Clare: Elsevier B.V., v. 183, p. 20-26, 2012.
ISSN 0168-9452 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 20-26
Origin http://dx.doi.org/10.1016/j.plantsci.2011.10.018
Access rights Closed access
Type Article
Web of Science ID WOS:000299713300004
URI http://repositorio.unifesp.br/handle/11600/34593

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